摘要
开花是高等植物内部遗传因素和外界环境因素共同调节完成的复杂生命过程,是植物进入生殖生长从而具备遗传能力和繁殖能力的象征。使用PCR技术,以楸树(Catalpa bungei)混合芽cDNA为模板分别克隆CbuATX1,CbuATX1-like和CbuATX23个基因,并利用相关生物信息学软件对这3个基因编码的蛋白质结构进行预测,同时对基因的启动子序列进行顺式作用元件分析,通过qRT-PCR技术检测3个基因在楸树混合芽不同发育时期的表达量。结果表明:CbuATX1的CDS全长为726 bp,编码241个氨基酸,存在跨膜运输结构,属于HMA蛋白家族,与芝麻(Sesamum indicum)、甜菜(Beta vulgaris)亲缘关系较近。CbuATX1-like的CDS全长为801 bp,编码266个氨基酸,存在跨膜运输结构,属于HMA蛋白家族,与胡萝卜(Daucus carota)、欧洲橄榄(Olea europaea)亲缘关系较近。CbuATX2的CDS全长为1554 bp,编码517个氨基酸,不存在跨膜运输结构,属于PWWP蛋白家族,与马尾草(Erythranthe guttatus)亲缘关系较近。这3个基因启动子均含有多个真核生物启动子的基本元件,如CAAT-box和TATA-box等,此外,还含有光响应、低温响应、生长素响应,以及与干旱诱导相关的MYB结合位点等3个基因与光响应密切相关,还可能参与外界环境胁迫响应等过程。qRT-PCR结果显示,上述3个基因在1年生普通楸树无性系9-1和突变株系-百日花楸树不同发育时期的表达量呈现显著差异。通过以上研究以期能够进一步阐述百日花楸树开花的性状,为楸树开花机制的研究和楸树的定向遗传改良提供理论支持。
Flowering is a complex life process that is regulated by both genetic factors and environmental factors in higher plants.It is a symbol of plants genetic and reproductive ability after it enters into reproductive growth.The genes of CbuATX1,CbuATX1-like and CbuATX2 were cloned respectively by PCR with cDNA as a template from Catalpa bungei flower bud,and the related bioinformatics software was used to predict the protein structure and subcellular location of these three genes respectively.The three genes promoter sequence were analyzed in cis-acting elements,and the expression levels of the three genes in different stages of C.bungei flower bud development were detected by qRT-PCR.The results showed that the CDS of CbuATX1 was 726 bp,encoding 241 amino acids with transmembrane transport structure.The protein was belonged to the HMA protein family and closely related to Sesamum indicum and Beta vulgaris.The CDS of CbuATX1-like was 801 bp.encoding 266 amino acids with transmembrane transport structure.The protein was belonged to the HMA protein family,which was closely related to Daucus carota and Olea europaea.The CDS of CbuATX2 was 1554 bp,encoding 517 amino acids with no transmembrane transport structure.The protein was belonged to the PWWP protein family and closely related to Erythranthe guttatus.The promoters of three genes contained several basic elements of eukaryotic promoters respectively,such as CAAT box and TATA box.In addition,they also contained light response factors,low temperature response factors and biological activity elements.The results of qRT-PCR showed that the expression levels of the three genes were significantly different in different developmental stages.It was hoped to further elaborate the flowering characteristics of C.bungei,and provided theoretical support for the flowering mechanism and directional genetic improvement of C.bungei.
作者
赵佳明
樊二勤
刘轶
王智
王军辉
曲冠证
Zhao Jiaming;Fan Erqin;Liu Yi;Wang Zhi;Wang Junhui;Qu Guanzheng(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;Institute of forestry,Chinese Academy of Forestry Sciences,State Key Laboratory of grassland forest cultivation,Catalpa National Innovation Alliance,Beijing 100091;China Academy of inspection and quarantine,Beijing 100176)
出处
《植物研究》
CAS
CSCD
北大核心
2022年第1期47-61,共15页
Bulletin of Botanical Research
基金
国家重点研发计划课题“楸树良种选育与高效培育技术研究”(2017YFD0600604)。
