摘要
目的研究微小RNA-106-3p(miRNA-106-3p)、微小RNA-654-5p(miRNA-654-5p)与结直肠癌(CRC)肝转移患者术后复发的关系。方法选取泰州市人民医院2014年12月至2017年12月收治的110例CRC肝转移患者,均行手术治疗。采用实时定量聚合酶链反应(RT-qPCR)检测两组患者miRNA-106-3p、miRNA-654-5p的表达水平。随访3年,5例失访,余105例根据复发情况分为复发组(30例)、未复发组(75例),比较两组手术前后的miRNA-106-3p、miRNA-654-5p表达水平。应用单因素分析及Logistic回归分析可能影响CRC肝转移术后复发的因素。结果手术前,两组miRNA-106-3p、miRNA-654-5p表达水平差异无统计学意义(P>0.05)。手术后,两组miRNA-106-3p、miRNA-654-5p表达均上调,且复发组高于未复发组,差异有统计学意义(P<0.05)。单因素分析结果显示,复发组与未复发组肝转移病灶分布、肝转移瘤范围、术后miRNA-106-3p及miRNA-654-5p表达水平差异有统计学意义(P<0.05)。两组性别、年龄、吸烟饮酒史、原发肿瘤位置及分化程度、脉管瘤栓、TNM分期、手术方式等差异无统计学意义(P>0.05)。Logistic回归分析显示,肝转移病灶双叶分布、肝转移瘤位于全肝、术后miRNA-106-3p、miRNA-654-5p高表达是影响CRC肝转移术后复发的危险因素。结论术后miRNA-106-3p、miRNA-654-5p表达上调可辅助诊断CRC肝转移患者术后是否出现复发现象,具有较高的临床价值。
Objective To study the relationship between microRNA-106-3 p(miRNA-106-3 p),microRNA-654-5 p(miRNA-654-5 p)and postoperative recurrence of colorectal cancer(CRC)patients with liver metastases.Methods A total of 110 patients with liver metastases from CRC who were admitted to the Taizhou People’s Hospital from December 2014 to December 2017 were selected for surgical treatment.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression levels of miRNA-106-3 p and miRNA-654-5 p in the two groups.After 3 years of follow-up,5 cases were lost to follow-up,and the remaining 105 cases were divided into recurrence group(30 cases)and non-recurrence group(75 cases)according to the recurrence status,the expression levels of miRNA-106-3 p and miRNA-654-5 p before and after the operation were compared between the two groups.Using univariate analysis and Logistic regression analysis may affect the factors that affect the recurrence of CRC after liver metastasis.Results Before surgery,there was no significant difference in the expression levels of miRNA-106-3 p and miRNA-654-5 p between the two groups(P>0.05).After surgery,the expressions of miRNA-106-3 p and miRNA-654-5 p were up-regulated in the two groups,and the expressions of miRNA-106-3 p and miRNA-654-5 p in the relapsed group were higher than those in the non-relapsed group,and the difference was statistically significant(P<0.05).The results of univariate analysis showed that there were statistically significant differences in the distribution of liver metastases,the extent of liver metastases,and the expression levels of miRNA-106-3 p and miRNA-654-5 p between the relapsed group and the non-relapsed group(P<0.05).There were no significant differences between the two groups in gender,age,smoking and drinking history,primary tumor location and degree of differentiation,vascular tumor thrombus,TNM staging,and surgical methods(P>0.05).Logistic regression showed that the distribution of liver metastases in two lobes,liver metastases located
作者
李杰
卢佳姝
LI Jie;LU Jiashu(Department of Gastroenterology,Taizhou People's Hospital,Taizhou 225300,China;Department of Pharmacy,Taizhou People's Hospital,Taizhou 225300,China)
出处
《中国肿瘤外科杂志》
CAS
2021年第6期606-610,共5页
Chinese Journal of Surgical Oncology
基金
2019年度江苏省《333工程》科研项目(BRA2019193)。
