摘要
类腐殖质(humic-like substance,HULIS)是一类水溶性、相对分子量高的有机混合物,常见于雾滴、云滴、积雪和大气颗粒物(PM)中.本文主要综述了大气PM中HULIS的提取和分析方法、毒理性、吸光特性和光敏性,重点探讨了PM中有机物和过渡金属(尤其是Fe离子)产生活性氧化性物种(reactive oxygen species, ROS)从而破坏DNA的致毒机理,进一步提出HULIS(含有可逆的氧化还原位点)单独或与过渡金属螯合成有机-金属配体形成ROS的机理.最后对未来大气HULIS的研究方向进行了展望,指出今后应该加强大气HULIS、金属等通过细胞内催生ROS的测定及细胞毒性相关的研究,并关注HULIS的化学结构、分子组分等的定量分析,更好地揭示物质结构与细胞毒性之间的作用机制和关系,以期为大气HULIS的健康影响提供支持.
Humic-like substances are a kind of water-soluble, high molecular weight organic mixtures, which usually exist in fog droplets, cloud droplets, snow and atmospheric particulate matters(PM). This paper mainly summarize extraction and isolation, analytical methods,toxicological property, absorbing-light characteristic and photosensitivity, with emphasis on exploring toxic mechanism on DNA damage via generation of reactive oxygen species(ROS) from organic matter and transition metals(especially iron), and further put forward ROS formation mechanism via individual HULIS or HULIS chelating with metal to form organic-metal complexes.At last, the research direction of ambient particle HULIS(reversible redox-active sites) in the future is prospected. It was pointed out that we should highlight intracellular ROS determination and cytotoxicity from atmospheric HULIS and metals, with more attention on quantitation on chemical structure, molecular composition. On the Basis of above study, we can better obtain driving mechanism and correlation between matter structure and cytotoxicity, and try to provide insights into the health risks caused by atmospheric HULIS.
作者
李旭东
孙宁
陶冶
陈彦彤
叶招莲
盖鑫磊
LI Xudong;SUN Ning;TAO Ye;CHEN Yantong;YE Zhaolian;GE Xinlei(School of Chemical and Environmental Engineering,Jiangsu University of Technology,Changzhou,213001,China;Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control,School of Environmental Science and Engineering,Nanjing University of Information Science&Technology,Nanjing,210044,China)
出处
《环境化学》
CAS
CSCD
北大核心
2021年第12期3787-3802,共16页
Environmental Chemistry
基金
江苏省自然科学基金(BK20181476,BK20181048)
国家自然科学基金(21976093)
江苏省普通高校专业学位研究生实践创新计划项目(XSJCX20_05,SJCX20_1030,XSJCX20_07)
江苏省大气环境监测与污染控制高技术研究重点实验室开放基金(KHK1904)资助。
关键词
类腐殖质
分离提取
吸光性
细胞毒性
活性氧化性物种
humic-like substances
extraction and isolation
light-absorbing characteristic
cytotoxicity
reactive oxygen species
