摘要
The ancient tea plant,as a precious natural resource and source of tea plant genetic diversity,is of great value for studying the evolutionary mechanism,diversification,and domestication of plants.The overall genetic diversity among ancient tea plants and the genetic changes that occurred during natural selection remain poorly understood.Here,we report the genome resequencing of eight different groups consisting of 120 ancient tea plants:six groups from Guizhou Province and two groups from Yunnan Province.Based on the 8,082,370 identified high-quality SNPs,we constructed phylogenetic relationships,assessed population structure,and performed genome-wide association studies(GWAS).Our phylogenetic analysis showed that the 120 ancient tea plants were mainly clustered into three groups and five single branches,which is consistent with the results of principal component analysis(PCA).Ancient tea plants were further divided into seven subpopulations based on genetic structure analysis.Moreover,it was found that the variation in ancient tea plants was not reduced by pressure from the external natural environment or artificial breeding(nonsynonymous/synonymous=1.05).By integrating GWAS,selection signals,and gene function prediction,four candidate genes were significantly associated with three leaf traits,and two candidate genes were significantly associated with plant type.These candidate genes can be used for further functional characterization and genetic improvement of tea plants.
基金
This work was supported by the Technology Creation Center of Guizhou Tea Industrialization(Qiankezhongyindi[2017]4005)
Guizhou Tea Industrial System-Function Laboratory of Tea Nutrition and Cultivation[K20-68-006]
Research on Key Technologies of the Quality Improvement of White,Yellow,and Purple Varieties(Qiankehe Platform Talent[2019]5651)
Screening and evaluation of tea germplasm resources with high EGCG in Guizhou based on SSR molecular marker technology(Qiankehe LH word[2017]No.7269).
