摘要
目的:探讨血浆miRNA-190和miRNA-197在肺血栓栓塞症(PTE)患者中表达水平及辅助诊断价值。方法:选择2017年7月至2019年6月苏州市第九人民医院PTE患者30例为PTE组,同期匹配心肌梗死患者45例为心肌梗死组,健康体检者45例为正常对照组。采用实时荧光定量PCR(qRT-PCR)检测血浆miRNA-190和miRNA-197在所有受试者外周血中的表达水平,并分析其在3组中的表达量差异。应用受试者工作特征曲线(ROC曲线)及曲线下面积(AUC)(95%CI)评估两者单独或联合检测作为PTE诊断指标的意义。结果:血浆miRNA-190在3组中的相对表达量分别为3.01±2.01,1.80±1.02,1.18±0.67;其在PTE组血浆中表达量较心肌梗死组及正常对照组高(t=3.602,t=5.814,P均<0.05)。血浆miRNA-197在3组中相对表达量分别为4.82±3.80,1.88±1.50,1.38±0.83;其在PTE组血浆中表达量较心肌梗死组及正常对照组高(t=4.791,t=5.886,P均<0.05)。作为PTE的诊断指标,血浆miRNA-190的最佳临界值为1.598,敏感度为75.56%,特异度为80.00%,AUC为0.7844,95%CI为0.6858~0.8831;血浆miRNA-197最佳临界值为2.40,敏感度为75.56%,特异度为86.67%,AUC为0.7931,95%CI为0.6870~0.8991。血浆miRNA190和miRNA-197联合检测诊断PTE的敏感度为80.00%,特异度为93.33%,AUC为0.9136,95%CI为0.8516~0.9756;均高于两者单独检测。结论:血浆miRNA-190和miRNA-197在PTE患者外周血血浆中表达量明显升高,两者联合检测敏感度和特异度更高,有助于PTE的辅助诊断。
Objective:To study the diagnostic value of quantitative detection of plasma miRNA-190 and miRNA-197 in patients with pulmonary thromboembolism(PTE).Methods:Totally,30 patients diagnosed with PTE admitted to Respiratory Medicine of Suzhou Ninth People's Hospital from July 2017 to June 2019 were enrolled in this study and plasma was collected.A total of 45 patients with myocardial infarction(MCI)and 45 healthy participants were recruited as the control group.qRT-PCR was used to detect the relative expression level of miRNA-190 and miRNA-197.The diagnostic value was evaluated by receiver operating characteristic(ROC)curves and the area under the ROC curve(AUC)(95%CI).Results:The plasma level of miRNA-190 in PTE group,MCI group and healthy group was 3.01±2.01,1.80±1.02 and 1.18±0.67 respectively.The plasma level of miRNA-190 in PTE group was significantly higher than in MCI group and healthy group(t=3.602,t=5.814,P all<0.0001).The plasma level of miRNA-197 in PTE group,MCI group and healthy group was 4.82±3.80,1.88±1.50,1.38±0.83 respectively,and the relative expression of miRNA-197 in PTE group was significantly higher than that in the other two groups(t=4.791,t=5.886,P all<0.05).As the diagnostic indicator,the sensitivity and specificity of miRNA-190 were 75.56%and 80%respectively,with the AUC being 0.7844(95%CI:0.6858-0.8831).The sensitivity and specificity of miRNA-197 was 75.56%and 86.67%and the AUC was 0.7931(95%CI:0.6870-0.8991).However,the combined detection of miRNA-190 and miRNA-197 showed the sensitivity and specificity of 80.00%and 93.33%respectively,and the AUC was 0.9136(95%CI:0.8516-0.9756).Conclusion:The relative expressions of miRNA-190 and miRNA-197 in PTE patients were significantly higher than those in MCI group and healthy group.Combined detection of miRNA-190 and miRNA-197 shows higher sensitivity and specificity,which is more conducive to the diagnosis of PTE.
作者
周晓婷
吴巧珍
徐瑞
胡晓蕴
董凌云
ZHOU Xiao-ting;WU Qiao-zhen;XU Rui;HU Xiao-yun;DONG Ling-yun(Department of Respiratory Medicine,Suzhou Ninth People's Hospital,Jiangsu Suzhou 215200,China)
出处
《内科急危重症杂志》
2021年第5期397-400,共4页
Journal of Critical Care In Internal Medicine
基金
苏州市第九人民医院科研基金项目(院201726、YK202109)。
