摘要
目的:采用双乳化溶剂蒸发法,优化无籽刺梨多糖纳米粒制备工艺,并对其进行表征。方法:采用响应面法以内水相W1和有机相O的比例、初级乳PE和外水相W2的比例以及泊洛沙姆P188的浓度为自变量,纳米粒的包封率为响应值,对制备工艺进行优化。结果:最佳制备工艺为W_(1)和O的比例为1:8,PE和W_(2)的比例为1:7,泊洛沙姆P188的浓度为0.1%(W/V)。该条件下制备的纳米粒粒径为219.14±2.67 nm,多分散系数为0.103±0.001,Zeta电位为-17.2±1.76 mV,包封率为95.08±0.90%,载药量为11.11±0.25%。透射电镜观察其呈均匀分布的球形,且表面光滑。结论:采用双乳化溶剂蒸发法制备的无籽刺梨多糖纳米粒包封率高、均匀圆整,为无籽刺梨多糖制剂的研发提供实验基础。
Objective:To optimize the preparation process of Rose sterilis D.Shi polysaccharide nanoparticles and characterize them by using double emulsification solvent evaporation method.Methods:In response surface methodology,the ratio of aqueous phase W_(1) to organic phase O,the ratio of primary emulsion PE to aqueous phase W_(2),and the concentration of poloxamer P188 were used as independent variables,and the encapsulation rate of nanoparticles was used as response value to optimize the preparation process.Results:The optimum preparation conditions were as follows:the ratio of W_(1) to O was 1:8,the ratio of PE to W_(2) was 1:7,and the concentration of poloxamer P188 was 0.1%(W/V).Under these conditions,the particle size of nanoparticles prepared was 219.14±2.67 nm,the polydispersion coefficient was 0.103±0.001,the Zeta potential was-17.2±1.76 mV,the encapsulation efficiency was 95.08±0.90%,and the drug loading was 11.11±0.25%.Transmission electron microscopy showed that the surface was smooth and spherical with uniform distribution.Conclusion:The Rose sterilis S.D.Shi polysaccharide nanoparticles prepared by the double emulsification solvent evaporation method have high encapsulation efficiency,uniformity and smoothness,and provide an experimental basis for the development of Rose sterilis polysaccharide preparations.
作者
付阳洋
杨敏
汤陆扬
杨培昕
彭黔荣
谢有超
Fu Yangyang;Yang Min;Tang Luyang;Yang Peixin;Peng Qianrong;Xie Youchao(School of Pharmacy,Guizhou University,Guiyang 550025,China;Technical Center of China Tobacco Guizhou Industry Co.,Ltd.,Guiyang 550009,China;School of Chemistry and Chemical Engineering,Guizhou University,Guiyang 550025,China)
出处
《中国野生植物资源》
CSCD
2022年第1期13-19,共7页
Chinese Wild Plant Resources
基金
国家自然科学基金项目(21562014)
贵州中烟科技项目(黔烟工技【2015】8号)。
关键词
无籽刺梨多糖
纳米粒
包封率
响应面
表征
Rose sterilis polysaccharide
Nanoparticles
Encapsulation efficiency
Response surface
Characterization
