摘要
以广东始兴枇杷叶为研究对象,以其总黄酮提取率为指标,探究了料液比、超声时间、乙醇体积分数、超声温度等因素对枇杷叶总黄酮提取率的影响,并利用Box-Behnken试验设计优化了提取工艺。结果表明,始兴枇杷叶总黄酮提取的最佳工艺是:超声温度64℃、超声时间54 min、乙醇体积分数62%、料液比1∶26 g/mL。在此条件下,总黄酮的实际提取率达3.68%,为广东始兴枇杷叶的综合开发利用提供了一定的科学参考。
This paper takes the loquat leaves in Shixing as the research object,takes the extraction rate of total flavonoids as the index,and explores the effects of material-liquid ratio,ultrasonic time,ethanol volume fraction,ultrasonic temperature and other factors on the extraction rate of total flavonoids from loquat leaves,and optimizes the extraction process by Box-Behnken design.The results showed that the optimum extraction process of total flavonoids from Shixing loquat leaves was as follows:ultrasonic temperature 64℃,ultrasonic time 54 mins,ethanol volume fraction 62%,and solid-liquid ratio 1∶26 g/mL.Under these conditions,the actual extraction rate of total flavonoids is 3.68%,which can provide scientific reference for the development and utilization of loquat leaves in Shixing,Guangdong.
作者
刘慕妆
李紫宁
赵晨惠
范文静
陈礼培
LIU Mu-zhuang;LI Zi-ning;ZHAO Chen-hui;FAN Wen-jing;CHEN Li-pei(School of Food Science and Engineering,Foshan University,Foshan 528231,China;Guangdong Traditional Fermented Food Engineering Technology Research Center,Foshan 528231,China;Guangdong Food Safety Control Engineering Technology Research Center,Foshan 528231,China;Foshan Brewing Engineering Technology Research Center,Foshan 528231,China;Foshan Agricultural Biological Manufacturing Engineering Technology Research Center,Foshan 528231,China)
出处
《佛山科学技术学院学报（自然科学版）》
CAS
2022年第1期26-34,共9页
Journal of Foshan University(Natural Science Edition)
基金
国家自然科学基金资助项目(32072198)
广东省自然科学基金资助项目(2020A1515011444)
广东省教育厅重点领域专项资助(2020ZDZX1029,2020ZDZX104)
佛山市科技奖培育入库资助项目(2020001003715)。
关键词
枇杷叶
总黄酮
超声波辅助
响应面法
loquat leaves
total flavonoids
ultrasonic assistance
response surface method
