摘要
目的:采用高效液相色谱法(high performance liquid chromatography,HPLC)测定生、熟大黄中的5种游离蒽醌的含量及差异,探讨中药炮制前后物质基础的变化规律。方法:使用色谱柱为Thermo BDS HYPERSII C_(18)柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸(85∶15)水溶液为流动相,检测波长为256 nm,流速1.0 mL/min,柱温30℃,进样量10μL。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.0207~0.414(R^(2)=0.9999)、0.1721~3.442(R^(2)=0.9996)、0.0203~0.406(R^(2)=0.9999)、0.148~2.96(R^(2)=0.9998)、0.164~3.28(R^(2)=0.9993)的线性范围内呈良好线性关系;生大黄经炮制后,芦荟大黄素含量下降约8.26%,大黄酸下降约9.21%;而大黄素含量增加约16.44%,大黄酚增加约16.12%,大黄素甲醚增加约20.01%,说明炮制工艺对大黄各成分影响不同。结论:大黄炮制后,其5种游离蒽醌的含量受到不同程度的影响,该方法可作为大黄及其不同炮制品的质量控制方法。
Objective:To determine the contents and differences of five free anthraquinones in crude and processed Dahuang(Chinese rhubarb)using HPLC,and discuss the rules of material basis before and after the processing.Methods:Thermo BDS HYPERSII C_(18) Column(4.6mm×250mm,5μm)was used as the chroma tographic column,methanol-0.1%phosphoric acid(85:15)aqueous solution as the mobile phase,detection wavelength was 256nm,flow rate 1.0mL/min,column temperature 30℃,sample size 10μL.Results:Aloe emodin,rhein,emodin,chrysophanol,emodin methyl ether showed better linear relationship in the range between 0.0207 and 0.414(R^(2)=0.9999),0.1721 and 3.442(R^(2)=0.9996),0.0203 and 0.406(R^(2)=0.9999),0.148 and 2.96(R^(2)=0.9998),0.164 and 3.28(R^(2)=0.9993)respectively;after processing,the contents of aloe emodin decreased by about 8.26%,the contents of rhein decreased by about 9.21%;while the contents of emodin increased by around 16.44%,the contents of chrysophanol by about 16.12%and emodin methyl ether by about 20.01%,which demo-nstrated that the processing showed different influences on all the ingredients of Dahuang.Conclusion:After processing,the contents of five free anthraquinones are affected to varying degrees,and the method could be used for quality control of Dahuang and its processed products.
作者
张如
魏江存
马家宝
杨正腾
王成龙
ZHANG Ru;WEI Jiangcun;MA Jiabao;YANG Zhengteng;WANG Chenglong(Maternity and Child Health Care Hospital of Guangxi Zhuang Autonomous Region,Nanning 530003,China;Guangxi International Zhuang Medicine Hospital;The First Affiliated Hospital of Guangxi University of Chinese Medicine)
出处
《西部中医药》
2021年第12期25-31,共7页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81360524)
2019年度广西高校中青年教师科研基础能力提升项目(2019KY0341)
广西中医药大学青年基金项目(2019QN036)
广西自然科学基金(2014GXNSFAA 118208)
国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室[国学基金中医药发2009(21号)]。
