摘要
采用益生菌植物乳杆菌LP21合成纳米硒,为抑制水产养殖中溶藻弧菌感染提供一种新的方法。通过植物乳杆菌LP21绿色合成纳米硒,对所得纳米硒进行透射电镜形貌及能谱分析,粒径仪测定粒径,红外光谱分析纳米硒表面生物分子的分布,并研究纳米硒对溶藻弧菌的抑菌活性。结果表明,植物乳杆菌LP21还原的纳米硒呈球形,分散性良好;纯化的纳米硒平均尺寸为184.6 nm,其表面包裹蛋白、多糖等生物分子,呈无定形态;纳米硒对溶藻弧菌、大肠杆菌的抑菌活性较强,对溶藻弧菌的最小抑菌浓度为286μg/mL,在亚抑制剂量下对溶藻弧菌的生长、运动性、生物膜有显著抑制,并引起菌体细胞形态发生变化,此抑菌研究为纳米硒在水产养殖业感染菌防治中的应用提供依据。
Nano-selenium synthesized by the probiotic bacterium Lactobacillus plantarum LP21 was analyzed by transmission electron microscopy(TEM) and energy spectroscopy, and its particle size was measured by a particle size analyzer. Additionally, biomolecule distribution on the surface of nano-selenium were analyzed by Fourier transform infrared(FTIR) spectroscopy, and the antibacterial activity of nano-selenium against Vibrio alginolyticus was tested. The results showed that the nano-selenium was spherical in shape and well dispersed;the average particle size of the purified nanoselenium was 184.6 nm, and its surface was covered with proteins, polysaccharides and other biological molecules, showing an amorphous form. The antibacterial activity of nano-selenium against V. alginolyticus and Escherichia coli was strong,and the minimum inhibitory concentration(MIC) against V. alginolyticus was 286 μg/mL. The growth, motility and biofilm of V. alginolyticus were significantly inhibited by the nano-selenium at sub-inhibitory doses, and the cell morphology was changed as well. The results of this study provide a basis for its application to control pathogenic infection in aquaculture.
作者
王丽红
杨辉
苏文
孔阳
WANG Lihong;YANG Hui;SU Wen;KONG Yang(School of Food and Biological Engineering,Shaanxi University of Science&Technology,Xi’an 710021,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第2期217-223,共7页
Food Science
基金
陕西省科技厅技术创新引导专项(2017CG-003)
陕西省重点研发计划项目(2018NY-043)。
关键词
植物乳杆菌
纳米硒
溶藻弧菌
结构表征
抑菌活性
Lactobacillus plantarum
nano-selenium
Vibrio alginolyticus
structural characterization
antibacterial activity
