摘要
选取黄河口潮间带有植被覆盖和无植被覆盖两个区域采集柱状沉积物,利用实时荧光定量PCR技术和高通量测序技术分析有无植被覆盖沉积物中的细菌群落特征和功能差异,探究影响潮间带细菌群落结构的主要环境因子。结果显示,细菌丰度在有植被区域大于无植被区域。沉积物中细菌群落丰富度和多样性在有植被区域随深度的增加而增加,而在无植被区域其最高值出现在沉积物中层(14—16 cm)。两个区域在门分类水平上以变形菌(Proteobacteria)、绿弯菌(Chloroflexi)、放线菌(Actinobacteria)和酸杆菌(Acidobacteria)为主;属分类水平上的优势类群为芽孢杆菌(Bacillus)、苍白杆菌(Ochrobactrum)、拟无枝菌酸菌(Amycolatopsis)和鞘脂单胞菌(Sphingomonas)等。相关性分析发现盐度和亚硝酸盐浓度对细菌群落多样性和丰富度影响显著。功能预测分析表明,有植被区域沉积物细菌在氨基酸代谢、膜运输和碳水化合物代谢方面功能活跃,而无植被区域细菌则在核酸复制和修复、能量代谢过程方面更为活跃。
In this study,the sediment cores were collected from two areas with or without vegetation covering in the intertidal zone of the Yellow River estuary,in which the characteristics and functional differences of bacterial community were analyzed based on real-time fluorescent quantitative PCR and high-throughput sequencing.The mainly environmental influencing factors were also explored.Results showed that the bacterial abundance from the vegetation area was higher than that from the vegetation-free area.The richness and diversity of bacterial community increased with the depth in the vegetation area,while they were the highest in the sample of middle layer(14—16 cm)in the vegetation-free area.The shared dominant phyla in both areas were Proteobacteria,Chloroflexi,Actinobacteria and Acidobacteria.The dominant genera were Bacillus,Ochrobactrum,Amycolatopsis,Sphingomonas and so on.The results of correlation analysis showed that salinity and nitrite concentration were the main influencing factors of the richness and diversity of bacterial community.The functional prediction analysis showed that the processes of bacterial membrane transport,amino acid metabolism and carbohydrate metabolism in the vegetation area were more active,while the processes of bacterial DNA replication,repairing and energy metabolism in the vegetation-free area were more active.
作者
尹霞
李思琦
尚天微
江雪艳
甄毓
YIN Xia;LI Siqi;SHANG Tianwei;JIANG Xueyan;ZHEN Yu(College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China;Key Laboratory of Marine Environment and Ecology,Ministry of Education,Qingdao 266100,China;College of Marine Life Science,Ocean University of China,Qingdao 266003,China;College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China)
出处
《生态学报》
CAS
CSCD
北大核心
2021年第21期8496-8506,共11页
Acta Ecologica Sinica
基金
国家自然科学基金项目(41876077)
青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室青年人才培育项目(LMEES-YTSP-2018-02-06)
中央高校基本科研业务项目(201962003)。
关键词
黄河口
潮间带
细菌
群落结构
Yellow River estuary
intertidal zone
bacteria
community structure
