摘要
利用AFB1免疫亲和柱对苦荞籽进行前处理,使用高效液相色谱仪测定AFB1的质量浓度,建立了一种检测苦荞籽中AFB1质量分数的检测方法。采用该方法测定了凉山州不同区域苦荞籽中AFB1的质量分数。结果表明:新建方法的检测限为0.2 ng/mL,精密度良好,添加回收率平均为85.87%。利用此法测定了凉山州不同区域的苦荞籽,在凉山州不同地区收集的苦荞籽中均未检出AFB1。
In order to establish a feasible method for monitoring aflatoxin B1 in Tartary buckwheat seeds,aflatoxin B1 immunoaffinity column combined with high performance liquid chromatography is used to determine the content of aflatoxin B1 in Tartary buckwheat seeds.The standard curve was drawn.The detection limit,and added recovery of the method were determined.The results show that the detection limits of the new method is 0.20 ng/mL,the precision is good,and the average recovery rate was 85.87%.The aflatoxin B1 contents of Tartary buckwheat seeds in different areas of Liangshan Prefecture are detected.The results show that the seeds of Tartary buckwheat collected from different areas of Liangshan Prefecture do not contain aflatoxin B1.
作者
刘恒
王安虎
刘晓燕
LIU Heng;WANG Anhu;LIU Xiaoyan(School of Agricultural Science,Xichang University,Xichang,Sichuan 615013,China)
出处
《西昌学院学报（自然科学版）》
2021年第4期6-9,共4页
Journal of Xichang University(Natural Science Edition)
基金
四川省科技厅项目(2018JY0397)。
关键词
苦荞种子
黄曲霉毒素B1
高效液相色谱法
免疫亲和柱
Tartary buckwheat seed
Aflatoxin B1
high performance liquid chromatography
immunoaffinity column
