摘要
植物中糖基转移酶(UDP-glycosyltransferases,UGTs)糖化各种植物激素和代谢物以应对生物和非生物胁迫.对龙眼UGTs(DlUGTs)家族进行全基因组鉴定,对各成员理化性质、系统发育进化树、基因结构、染色体定位、保守基序、全基因组共线性进行预测分析,并结合转录组对DlUGTs在龙眼体胚发育胚性愈伤组织(EC)、不完全胚性紧实结构(ICpEC)和球形胚(GE)3个阶段的表达模式进行分析.结果显示,DlUGTs家族共有167个成员.系统发育进化树分析发现这些成员分布于15个组中.蛋白保守基序分析发现所有成员均包含保守motif 1(PSPG-box)且同组成员的motif分布高度相似.对DlUGTs家族成员的基因结构进行分析,发现其内含子数目在0-4之间.启动子顺式作用元件分析发现,各成员启动子区包含众多响应生物与非生物胁迫的作用元件,如光、温、激素等.染色体定位分析发现DlUGTs家族成员分布在14条染色体上,33个成员定位在chr14.对DlUGTs在EC-ICpEC-GE阶段的表达模式分析发现共138(83%)个成员在3个阶段检测到表达量,其中7个成员在EC-ICpEC-GE阶段呈持续上调表达.本研究通过预测分析发现DlUGTs家族成员具备功能多样性,可能在龙眼应对胁迫、细胞代谢及体胚发育过程中发挥作用,结果可为UGT关键基因的进一步筛选和表征提供理论依据.
UDP-glycosyltransferases(UGTs) glycosylate various plant hormones and metabolites to respond to biotic and abiotic stresses. In this study, we conducted a genome-wide identification of the longan(Dimocarpus longan Lour.) UGT(hereafter, DlUGT) family. The physicochemical properties, phylogenetic tree, gene structure,chromosomal location, conservative motif, and genome-wide collinearity were analyzed using bioinformatics methods. The expression patterns of DlUGTs in three early somatic embryo developmental stages were analyzed in combination with the transcriptome, including embryogenic calluses(EC), incomplete compact proembryogenic calluses(ICpEC), and globular embryos(GE). The results revealed that the DlUGT family had 167 members and phylogenetic tree analysis distributed these members into 15 groups. Protein conservative motif analysis revealed that all members had a conservative motif 1(PSPG-box), and the motif distribution of each group was very similar. The gene structure of DlUGT family members was analyzed, and the number of introns were found to be between 0 and 4. The analysis of the cis-acting elements of the promoter revealed that each member’s promoter region contained many elements that responds to biotic and abiotic stresses, such as light, temperature, and hormones. The members of the DlUGT family were distributed on 14 chromosomes,and 33 members were located on chromosome 14. The expression pattern analysis revealed that a total of 138 members(83%) had their expression levels detected in three stages. In addition, analysis of the expression pattern of DlUGTs in the EC-ICpEC-GE stage revealed that 138 members(83%) had their expression levels detected in the three stages, of which seven members were continuously upregulated in the EC-ICpEC-GE stage. Through predictive analyses, this study found that DlUGT family members exhibit functional diversity and may play a role in the response of longan to stress, cell metabolism, and somatic embryo development. Finally,this study provides a theoretical ba
作者
刘蒲东
张舒婷
申序
李晓斐
赵桦
张梓浩
陈裕坤
林玉玲
陈振光
王晶
赖钟雄
LIU Pudong;ZHANG Shuting;SHEN Xu;LI Xiaofei;ZHAO Hua;ZHANG Zihao;CHEN Yukun;LIN Yuling;CHEN Zhenguang;WANG Jing;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Cash Crops,Shanxi Agricultural University,Fenyang 032200,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2021年第6期1626-1635,共10页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31572088)
福建省高原学科建设经费(102/71201801101)
福建农林大学科技创新专项基金项目(KFA19037A,CXZX2017314)
福建农林大学园艺学院优硕基金项目(102-1122YS010)资助。
关键词
龙眼
糖基转移酶
成员鉴定
功能预测
表达模式
Dimocarpus longan Lour.
UDP-glycosyltransferase
membership identification
function prediction
expression pattern
