摘要
随着集约化畜禽养殖业的迅猛发展,抗生素大量使用并通过施肥不断进入土壤环境,给农田生态系统安全带来巨大的负面效应.因此,开展抗生素污染土壤的修复具有重要的理论和实践意义.选用四环素(TC)作为抗生素模型药物,采用土培试验对模拟污染土壤中TC进行修复研究,考察不同修复基质(蚯蚓粪、菌糠和生物炭)及其与菌剂配施对土壤理化性质及微生物活性的影响.结果显示,TC对土壤养分及其相关性状指标均有促进作用.菌糠添加显著促进了土壤速效磷、碱解氮和阳离子交换量,增幅分别为252.66%,41.65%和23.44%;生物炭配施菌剂则显著促进了土壤有机质和速效钾含量,增幅分别为148.95%和111.69%. TC对土壤过氧化氢酶、脲酶及蔗糖酶活性均有一定抑制作用,其中对脲酶的抑制作用最强,抑制率变化范围为40.25%(90 d)-61.60%(60 d);而菌糠配施菌剂对这3种酶的活性修复效果最明显. TC对土壤微生物量C和N分别表现为抑制和激活作用,菌糠配施菌剂对土壤微生物量C和N增幅作用最明显.相关性分析表明,土壤理化性质与微生物活性存在显著相关关系,特别是pH和土壤养分(速效钾和速效磷等)的影响极为显著.添加不同修复基质一定程度上能缓解TC对土壤的毒害作用,特别是菌糠配施菌剂对土壤酶活性及微生物量C和N的提高效果最佳.
With the rapid development of the livestock and poultry breeding industry, the extensive use of antibiotics has resulted in significant negative effects on the security of farmland ecosystems because of their continuous entry into the soil environment through fertilization. Therefore, the remediation of antibioticcontaminated soils is of considerable theoretical and practical significance. In this study, tetracycline(TC)was selected as an antibiotic case study drug, and soil culture experiments were conducted to investigate the remediation of TC in simulated contaminated soil. The effects of different remediation substrates(wormcast, fungal chaff, and biochar) and their combinations with microbial inoculum were investigated on soil physicochemical properties and microbial activities. The results showed that TC promoted soil nutrients and its related properties. The addition of fungal chaff promoted an increase in available phosphorus, alkalihydrolyzable nitrogen, and cation exchange capacity by 252.66%, 41.65%, and 23.44%, respectively. Organic matter and available potassium were significantly increased by 148.95% and 111.69%, respectively, because of the addition of biochar with microbial inoculum. TC inhibited the activities of soil catalase, urease, and sucrase,among which the urease had the strongest inhibitory effect, with an inhibition rate ranging from 40.25%(90 d)to 61.60%(60 d). The effect of fungal chaff, combined with microbial inoculum on the activities of these three enzymes, was the most obvious. The addition of different remediation substrates could alleviate the toxicity of tetracycline to the soil, particularly when fungal chaff combined with microbial inoculum, was applied, which had the best effect on improving soil enzyme activity and microbial biomass C and N.
作者
秦俊梅
李嘉欣
宋建丽
运佳兴
熊华烨
刘奋武
张健
毕文龙
QIN Junmei;LI Jiaxin;SONG Jianli;YUN Jiaxing;XIONG Huaye;LIU Fenwu;ZHANG Jian;BI Wenlong(College of Resources and Environment,Shanxi Agricultural University,Taigu 030801,China;College of Resources and Environment,Southwest University,Chongqing 400716,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2021年第6期1500-1508,共9页
Chinese Journal of Applied and Environmental Biology
基金
山西省重点研发计划项目(201903D221015)
山西省青年科技研究基金项目(201901D211385)
山西省青年科技研究基金项目(201901D211353)资助。
关键词
修复基质
四环素
土壤养分
土壤酶活性
土壤微生物量碳
土壤微生物量氮
remediation substrate
tetracycline
soil nutrient
soil enzyme activity
soil microbial biomass C
soil microbial biomass N
