摘要
【目的】番茄斑萎病毒(TSWV)在全球范围内广泛发生,危害多种作物引起严重的经济损失,筛选应用具有高效抑制活性的天然产物是防控TSWV引起的病害有效途径之一。前期研究发现三列叶蟛蜞菊(Wedelia trilobata)中提取的贝壳杉烯酸(AHK)具有抑制TSWV侵染的活性,本研究探明其具体的抑制机制。【方法】通过测定接种TSWV前、后用AHK处理的烟草植株相关防御酶活性以及RT-qPCR检测抗性相关主要代谢通路关键转录因子基因表达量,解析AHK防御TSWV侵染的机制。【结果】AHK在病毒接种前后处理中能诱导超氧化物歧化酶(SOD)活性升高而抑制TSWV的侵染;同时AHK能诱导茉莉酸(JA)通路重要转录因子MYC2、NAC表达量升高,增强了系统获得抗性。【结论】AHK主要通过诱导SOD酶活升高以及诱导JA通路增强寄主系统抗性从而抑制TSWV的侵染,具有作为植物源农药研发的潜力,为TSWV引起的病毒病绿色防控积累基础。
【Objective】Tomato spotted wilt orthotospovirus(TSWV)had occurred worldwide and caused seriously economic losses on many crops.It was one of the efficient path via screening of natural products with high inhibited activity to prevention and control of the diseases caused by TSWV.Our previous study indicated that kaurenic acid(AHK)extracted from Wedelia trilobata has a high effect against TSWV infection.In this study,we want to prove up the mechanism of AHK inhibition.【Method】Detected the activities of defense enzymes and the expression of systemic acquired resistance(SAR)-related genes by RT-qPCR from the tobacco plants before and post inoculated TSWV and treatment AHK,and analyzed the mechanism of inhibited by AHK.【Result】AHK induced superoxide dismutase(SOD)activity rised and inhibited TSWV infection.Meanwhile,AHK induced the increasing of MYC2 and NAC expression levels which were important transcription factors of Jasmonic Acid(JA)pathway.【Conclusion】AHK mainly induced the increasing of SOD activity and JA signaling pathway to enhance host SAR for inhibiting TSWV infection,and AHK had potential for the research and development of botanical pesticides for bases of prevention and contrl the diseases caused by TSWV.
作者
赵立华
杨婷婷
邱润霜
何思洁
余萍
郑雪
张丽珍
张仲凯
ZHAO Li-hua;YANG Ting-ting;QIU Run-shuang;HE Si-jie;YU Ping;ZHENG Xue;ZHANG Li-zhen;ZHANG Zhong-kai(Biotechnology and Genetic Germplasm Resources Research Institute,Yunnan Academy of Agricultural Sciences/Key Lab of Agricultural Biotechnology of Yunnan Province/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation Ministry of Agriculture and Rural Affairs,Yunnan Kunming 650205,China;Garden and Horticulture College,Southwest Forestry University,Yunnan Kunming 650224,China)
出处
《西南农业学报》
CSCD
北大核心
2021年第12期2659-2665,共7页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金地区基金项目“圆叶肿柄菊素A诱导茉莉酸通路抑制TSWV侵染的分子机制”(31960531)
国家自然科学基金联合基金项目“植物布尼亚病毒诱导病理囊泡的机制及对病毒系统侵染的调控作用”(U1802235)
云南省基础研究面上项目“圆叶肿柄菊素A抑制番茄斑萎病毒复制、装配机制”(202101AT070269)
云南省“云岭学者”培养计划项目(2016-2020)。
关键词
番茄斑萎病毒
贝壳杉烯酸
超氧化物歧化酶
茉莉酸通路
系统抗性
Tomato spotted wilt orthotospovirus
Kaurenic acid
Superoxide dismutase
Jasmonic acid signaling pathway
Systemic acquired resistance
