摘要
对我国皱环球盖菇Stropharia rugosoannulata 20个主栽菌株进行了ISSR标记遗传多样性分析,使用的28个ISSR引物中22个具有多态性,UPGMA聚类分析显示遗传相似性水平在0.68–0.86之间,在0.72时可将菌株分为6个类群,类群间遗传差异较大。在PDA培养基上,除菌株Sr-03和Sr-05最适生长pH值为8.0外,其他菌株均为pH 5.0–6.0;在5–30℃的温度范围内,除菌株Sr-01、Sr-08、Sr-11在30℃时长速受到抑制外,其他菌株长速随温度升高而加快,但在35℃时仅有5个菌株在培养20d时具有活性;皱环球盖菇菌丝在以木屑为主的原种培养料中长速较慢,为0.73–1.08mm/d。在菌株的农艺性状比较中,菌株Sr-12的菇型比例最好、产量最高,生物学效率达98.09%,菌株Sr-08和Sr-12菇体硬度较大,菌盖颜色与其他菌株差异显著,菌株Sr-19和Sr-20的菌褶颜色较其他菌株差异较大。本研究筛选出了7个具有遗传差异的优异种质,产量高、抗逆性强,并在菇型、菌盖颜色、菌褶颜色等农艺性状上具有较大的差异,可为皱环球盖菇育种及遗传研究提供菌株选择。
Genetic diversity analysis was carried out by using ISSR markers in 20 main cultivars of Stropharia rugosoannulata in China.In 28 commonly used ISSR primers 22 were polymorphic.UPGMA cluster analysis showed the genetic similarity level was 0.68–0.86.Strains can be divided into six groups at 0.72 level,which indicate great genetic differences between groups.The optimum pH value for growth on PDA medium was 5.0–6.0,except for strains Sr-03 and Sr-05 which were at pH 8.0.The growth temperature ranged from 5℃to 30℃,except for strains Sr-01,Sr-08,and Sr-11 which stagnated at 30℃.The growth rate was increased with the increase of temperature,and only five strains were survival at 35℃in 20 days of cultivation.The mycelia of Stropharia rugosoannulata grows slowly in the compost using sawdust as main substrate,with growth rate of 0.73–1.08mm/d.Comparison of the agronomic characteristics among strains indicates that the strain Sr-12 has the best fruiting-body shape and the highest yield,with a biological efficiency of 98.09%.Sr-08 and Sr-12 strains have better fruiting-body hardness and significant difference in cap color as compared with other strains.The gill color of Sr-19 and Sr-20 differs from that of other strains.Combining the differences in genetic diversity,biological characteristics,and agronomic traits,this experiment screened out seven excellent germplasms with high yield and strong stress resistance,and they can be selected for breeding and genetic research of S.rugosoannulata.
作者
陈绪涛
章炉军
孙鹏
胡文君
王瑞娟
张美彦
魏云辉
王洪秀
胡佳
熊泽亚
CHEN Xu-Tao;ZHANG Lu-Jun;SUN Peng;HU Wen-Jun;WANG Rui-Juan;ZHANG Mei-Yan;WEI Yun-Hui;WANG Hong-Xiu;HU Jia;XIONG Ze-Ya(Institute of Agricultural Applied Microbiology,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi 330200,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Gao’an Public Inspection and Testing Center,Gao’an,Jiangxi 330800,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第12期3081-3095,共15页
Mycosystema
基金
财政部和农业农村部:国家现代农业产业技术体系(CARS-20)
江西省重点研发计划重点项目(20212BBF61002)
江西省重点研发计划一般项目(20212BBF63013)。
关键词
皱环球盖菇
遗传多样性
优异种质
育种
Stropharia rugosoannulata
genetic diversity
excellent germplasm
breeding
