摘要
目的:探索针刀、电针治疗对早期膝骨关节炎(KOA)兔模型关节软骨细胞结构及Ⅱ型胶原的表达情况。方法:30只新西兰兔按体质量随机分为空白组(6只)及KOA造模组(24只),KOA造模组应用改良Videman法制动4周建立早期KOA兔模型。干预结束后,对兔股骨内侧髁软骨进行免疫荧光双标染色(激光共聚焦显微镜观察),应用ELISA法检测兔血清、关节液CTX-2的含量。结果:①治疗后针刀组、电针组血清(P=0.091,P=0.657)、关节液(P=0.066,P=0.032)CTX-2含量降低。②治疗后针刀组、电针组细胞核(P=0.058,P=0.051)、F-actin骨架蛋白(P=0.184,P=0.220)和Ⅱ型胶原(P=0.168,P=0.236)IOD表达降低,但差异无统计学意义。结论:针刀、电针对早期KOA兔模型膝关节软骨细胞及Ⅱ型胶原蛋白代谢具有一定保护作用,但效果不如塞来昔布。
Objective:To explore the effects of acupotomy and electro-acupuncture on structure of articular chondrocyte and expression of typeⅡcollagen in rabbit model of early knee osteoarthritis(KOA).Methods:30 New Zealand rabbits were randomly divided into the blank group(n=6)and the model group(n=24).The KOA model was established by modified Videman method(immobilization for 4 weeks).After the interventions of acupotomy and electro-acupuncture,the rabbit femoral condyle cartilage was stained with double immunofluorescence and observed by laser confocal microscopy.The contents of CTX-2 in serum and in synovial fluid were detected by ELISA.Results:After the interventions,the contents of CTX-2 in serum(P=0.091,P=0.657)and in synovial fluid(P=0.066,P=0.032)were decreased in the acupotomy group and in the electro-acupuncture group.The IOD expressions of the nucleus(P=0.058,P=0.051),F-actin(P=0.184,P=0.220)and typeⅡcollagen(P=0.168,P=0.236)were decreased in the acupotomy group and in the electro-acupuncture group,but there were no statistical differences.Conclusion:Acupotomy and electro-acupuncture have certain protective effects on the articular chondrocytes and the metabolism of typeⅡcollagen in rabbits with early KOA,however the effects are not as effective as Celecoxib.
作者
史晓伟
王东峰
邓晓丰
于文静
赵颖
史术峰
SHI Xiaowei;WANG Dongfeng;DENG Xiaofeng;YU Wenjing;ZHAO Ying;SHI Shufeng(The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China)
出处
《针灸临床杂志》
2022年第1期59-64,共6页
Journal of Clinical Acupuncture and Moxibustion
基金
国家自然科学基金,编号:81804184
中国博士后科学基金,编号:2018M640098。
关键词
膝骨关节炎
针刀
电针
免疫荧光
Ⅱ型胶原蛋白
Knee osteoarthritis
Acupotomy
Electro-acupuncture
Immunofluorescence
TypeⅡcollagen
