摘要
目的:探讨不同年龄段小鼠应激造模前后的抑郁及焦虑样行为。方法:54只C57BL/6J小鼠分为正常对照(NC)组和慢性束缚应激(CRS)组,再根据年龄分为青年亚组、中年亚组、老年亚组,共6亚组,每亚组9只。通过检测各组小鼠的行为学改变,包括体质量、悬尾试验、强迫游泳试验、糖水偏好试验、开放旷场实验,观察小鼠抑郁样及焦虑样行为。结果:NC组中,与青年亚组相比,中年亚组小鼠直立次数减少(P<0.05),老年亚组小鼠体质量升高(P<0.05),悬尾、强迫游泳的不动时间下降(P<0.05),旷场中移动的总距离下降、直立次数减少(P<0.05);与NC组各亚组相比,CRS组对应各亚组的体质量下降(P<0.05),悬尾、强迫游泳的不动时间上升(P<0.05),糖水偏好率、旷场移动总距离、直立次数均下降(P<0.05),其中与CRS组青年亚组相比,老年亚组的悬尾、强迫游泳的不动时间升高(P<0.05),糖水偏好率下降(P<0.05),旷场直立次数下降(P<0.05)。结论:正常衰老状态下,小鼠年龄越小越容易出现绝望行为,年龄越大焦虑样行为越严重;应激衰老状态下,小鼠年龄越大,抑郁焦虑样行为程度越严重,特别是奖赏功能受损导致的快感缺失。
Objective:To explore the depressive-like and anxiety-like behavior of mice at different ages before and after modeling.Methods:A total of 54 C57BL/6J mice were divided into the normal control(NC)group and chronic restraint stress(CRS)group,and each group was divided into 3 subgroups according to age,including young subgroup,middle-aged subgroup,and old subgroup,for a total of 6 subgroups with 9 mice in each.The behavioral changes of mice in each group were studied through assessing the body weight,tail suspension test,forced swimming test,sugar preference test,and open field test to evaluate the depressive-like and anxiety-like behavior of the mice.Results:Within the NC group,compared with that of the young subgroup,the frequency of rearing behavior of the middle-aged subgroup was significantly decreased(P<0.05);the body weight of the old subgroup was significantly increased(P<0.05),the immobility time during tail suspension and forced swimming was significantly decreased(P<0.05),and the total distance traveled and frequency of rearing behavior in the open field were significantly decreased(P<0.05).Compared with that of the NC group,body weight of all subgroups in the CRS group was significantly decreased(P<0.05);immobility time during tail suspension and forced swimming was significantly increased(P<0.05);and sugar preference rate and the total distance traveled and frequency of rearing behavior in the open field were significantly decreased(P<0.05).Within the CRS group,compared to that of the young subgroup,the immobility time during tail suspension and forced swimming in the old subgroup was significantly increased(P<0.05);the sugar preference rate was significantly decreased(P<0.05);and the frequency of rearing behavior in the open field was significantly decreased(P<0.05).Conclusion:With normal aging,the younger the mice,the more likely they were to show behaviors of despair;the older the mice,the more severe were their behaviors of anxiety.With stress-related aging,the older the mice,the more serious was th
作者
孙思琦
陈国鹏
周恩奇
刘忠纯
SUN Si-qi;CHEN Guo-peng;ZHOU En-qi;LIU Zhong-chun(Department of Psychiatry,Renmin Hospital of Wuhan University,Wuhan 430060,China)
出处
《神经损伤与功能重建》
2022年第1期4-8,共5页
Neural Injury and Functional Reconstruction
基金
国家重点研发计划(No.2018YFC1314600)
国家自然科学基金(No.81771472)。
关键词
衰老
抑郁
焦虑
快感缺失
age
depression
anxiety
anhedonia
