摘要
为了解不同来源大麦对条纹病的抗性及遗传多样性,本研究采用“三明治法”通过人工接种大麦条纹病菌对91份大麦材料进行抗性评价,并通过31对SSR标记对91份大麦材料进行遗传多样性和群体结构分析。结果表明,人工接种大麦条纹病菌后共鉴定出4份免疫、6份高抗、33份抗病、43份感病和5份高感大麦材料;31对SSR标记共检测出等位基因238个,平均每对标记可检测到7.677个等位基因,等位基因数的变幅为2~19;主基因频率变化范围为0.236~0.951,平均值为0.394;基因多样性指数的变幅为0.094~0.871,平均值为0.667;多态性信息含量变幅为0.091~0.860,平均值为0.613;遗传相似系数变异范围为0.103~1.000,平均值为0.522;在遗传相似系数为0.783水平上可将参试材料聚为3个大类群,各大类分别包含86份、2份和3份材料;群体遗传结构分析表明,供试大麦材料分为3个亚群,每个亚群分别包含47份、33份和11份材料,且在91份材料中,Q>0.6的材料占总数的97.80%。本研究经抗病鉴定及分子标记结果综合分析,可为挑选抗病亲本辅助抗大麦条纹病优良品种的选育提供参考。
In order to detect the resistance genes of germplasm resources against barley stripe disease and understand their genetic diversity,in this study 91 barley accessions were inoculated with barley stripe disease pathogen by sandwich method,followed by the genetic diversity analysis using 31 polymorphic SSR markers.Of them,four accessions were identified to be complete resistance(free of diseased symptom),6 highly resistant,33 resistant,43 susceptible and 5 high susceptible.Genotyping by 31 SSR markers disclosed 238 alleles,with a mean of 7.677 alleles in each of markers and the range of 2 to 19 alleles.The frequency of major loci ranged from 0.236 to 0.951,with an average of 0.394.The gene diversity index ranged from 0.094 to 0.871,with an average of 0.667.PIC ranged from 0.091 to 0.860,with an average of 0.613,while GS ranged from 0.103 to 1.000,with an average of 0.522.This collection were divided into three groups at the genetic similarity coefficient level of 0.783,each containing 86,2 and 3 accessions,respectively.The population structure analysis suggested three groups,each including 47,33 and 11 materials,respectively.97.80%of 91 genotypes had Q value greater than 0.6.Gained from disease resistance identification and genotyping,this study provided reference for selecting parental lines in breeding of barley leaf stripe resistant varieties.
作者
郭铭
张金福
司二静
孙莉莎
魏建敏
刘海颖
乔岩
姚立蓉
汪军成
李葆春
杨轲
孟亚雄
马小乐
朱靖环
尚勋武
王化俊
GUO Ming;ZHANG Jin-fu;SI Er-jing;SUN Li-sha;WEI Jian-min;LIU Hai-ying;QIAO Yan;YAO Li-rong;WANG Jun-cheng;LI Bao-chun;YANG Ke;MENG Ya-xiong;MA Xiao-le;ZHU Jing-huan;SHANG Xun-wu;WANG Hua-jun(Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement and Germplasm Enhancement,Lanzhou 730070;College of Agronomy,Gansu Agricultural University,Lanzhou 730070;Gansu Provincial Farmer Education and Training Station,Lanzhou 730000;College of Agriculture and Forestry,Longdong University,Gansu Qingyang 745000;College of Life Sciences and Technology,Gansu Agriculture University,Lanzhou 730070;Institute of Crops and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2022年第1期72-82,共11页
Journal of Plant Genetic Resources
基金
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-05-04B-2)
甘肃省教育厅产业支撑计划项目(2021CYZC-12)
国家自然基金(31960426,32160496)
甘肃省科技重大专项计划(17ZD2NA016)
甘肃省青年科技基金计划(20JR5RA010)
甘肃农业大学科技创新基金-公招博士科研启动基金(GSAURCZX201706)
甘肃农业大学学科建设专项基金(GAU-XKJS-2018-082,GAUXKJS-2018-083)
甘肃省科技支撑计划项目(1604NKCA052)
甘肃省教育厅创新能力提升项目(2019A-053,2021A-055)。
关键词
大麦
大麦条纹病
抗性鉴定
遗传多样性
群体遗传结构
barley
barley leaf stripe
resistance identification
genetic diversity
population genetic structure
