摘要
土壤真菌是生态系统物质循环和能量流动的重要推动者。为探明西南喀斯特峰丛洼地不同森林类型土壤真菌群落结构及影响因素,选择了三个典型森林(灌木林、次生林、原生林)的土壤样品进行真菌ITS高通量测序。结果表明,灌木林和原生林土壤理化性质最为接近,养分含量较高。33个土壤样品中共获得2 013 410条有效序列,9 677个OTUs,分属于18个门、59个纲及946个属。三类森林土壤真菌均由子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、未分类真菌(unclassified Fungi)、被孢霉门(Mortierellomycota)和罗兹菌门(Rozellomycota)组成,其中,子囊菌门和担子菌门是三类森林土壤真菌的优势菌门,所占比例近80%。原生林和灌木林α多样性最高,具有最为接近的真菌群落组成和结构。LEfSe分析表明,各森林关键优势真菌类群各不相同,灌木林中的差异种有粪壳菌纲(Sordariomycetes),散囊菌(Eurotiomycetes、Eurotiales),丛赤壳科(Nectriaceae);次生林的差异种主要是伞菌纲(Agaricomycetes)和古根菌纲(Archaeorhizomycetes);而未分类的伞菌目(unclassified Agaricales)和丝盖伞菌(Inocybaceae、Inocybe)则在原生林起关键作用。相关分析发现土壤真菌与土壤理化因子显著相关且关系十分密切,冗余分析进一步表明土壤的交换性钙离子、镁离子、全钾、温度对真菌群落的分布作用较大,是影响喀斯特森林土壤真菌群落结构和分布的主导因子。
Soil fungi is an important promoters for material circulation and energy flow in ecosystems.In order to explore the community structure and influencing factors of soil fungi in different forest types in karst hills among depression in Southwest China,soil samples from three typical forests (shrub,secondary forest and primary forest) were selected for fungal ITS high-throughput sequencing.The results showed that the soil physical and chemical properties in shrub forest and primary forest were closest,with high nutrient content.A total of 2 013 410 valid sequences and 9 677 OTU were obtained from the 33 soil samples,which belonged to 18 phyla,59 classes and 946 genera.Soil fungi were composed of Ascomycota,Basidiomycota,unclassified fungi,Mortierellomycota,and Rozellomycota,in all the three forest types.Among them,Ascomycota and Basidiomycota were the dominant soil fungi in three forest types,which together accounted for nearly 80% of the total fungal abundance.The primary forest and shrub forest had the higher α diversity with the closest fungal community composition and structure.LEfSe analysis showed that the key dominant fungal groups were different in each forest.Specifically,the biomarkers were Sordariomycetes,Eurotiomycetes,Eurotiales,and Nectriaceae in the shrub forest,Agaricomycetes and Archaeorhizomycetes in the secondary forest,while unclassified Agaricales and Inocybaceae in the primary forest.Correlation analysis showed that soil fungi were closely related to soil physical and chemical factors.Redundancy analysis further showed that exchangeable Ca;,Mg;,total potassium and temperature had great effects on the soil fungal distribution of soil fungal,which were the dominant factors affecting the soil fungal community structure and distribution in karst forests.
作者
陈惠君
莫雅芳
封红梅
王家妍
曾馥平
宋同清
彭晚霞
杜虎
王枫
CHEN Hui-jun;MO Ya-fang;FENG Hong-mei;WANG Jia-yan;ZENG Fu-ping;SONG Tong-qing;PENG Wan-xia;DU Hu;WANG Feng(Key laboratory of Agro-ecological Processes in Subtropical Region,Institute of subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;Huanjiang Observation and Research Station for Karst Ecosystems,Chinese Academy of Sciences,Huanjiang,Guangxi 547100,China;University of Chinese Academy of Sciences,Beijing 100049,China;Guangxi Gaofeng State Owned Forest Farm,Nanning,Guangxi 530001,China)
出处
《农业现代化研究》
CSCD
北大核心
2021年第6期1146-1157,共12页
Research of Agricultural Modernization
基金
国家自然科学基金项目(31770495,31971487,42071073)。
