摘要
目的探讨乙型病毒性肝炎患者血清微小RNA-101(miR-101)、脂肪酸结合蛋白4(FABP4)mRNA、脂肪酸结合蛋白5(FABP5)mRNA水平与肝纤维化程度的相关性。方法选取2020年3月至2021年3月于河南大学第一附属医院确诊的96例乙型病毒性肝炎患者,纳入乙型肝炎组。选取同期该院健康体检者94例作为对照组。根据乙型病毒性肝炎患者肝纤维化程度将其分为S 0期(40例)、S_(1)期(28例)、S_(2)期(16例)、S_(3)期(12例)。采用实时荧光定量聚合酶链式反应(qRT-PCR)法检测血清miR-101、FABP4 mRNA、FABP5 mRNA水平。采用Pearson法分析乙型病毒性肝炎患者血清miR-101与FABP4 mRNA、FABP5 mRNA水平的相关性。采用Spearman法分析血清miR-101、FABP4 mRNA、FABP5 mRNA水平与乙型病毒性肝炎患者肝纤维化程度的相关性。结果乙型肝炎组血清FABP4 mRNA、FABP5 mRNA水平高于对照组,miR-101水平低于对照组(P<0.05)。血清FABP4 mRNA、FABP5 mRNA水平随着肝纤维化分期的增加而上升,miR-101水平随着肝纤维化分期的增加而下降(P<0.05)。乙型病毒性肝炎患者血清miR-101与FABP4 mRNA、FABP5 mRNA水平均呈负相关(r=-0.524、-0.590,P<0.05)。乙型病毒性肝炎患者肝纤维化程度与血清FABP4 mRNA、FABP5 mRNA均呈正相关(r=0.654、0.723,P<0.05),与miR-101呈负相关(r=-0.861,P<0.05)。结论血清miR-101、FABP4 mRNA、FABP5 mRNA水平与乙型病毒性肝炎患者肝纤维化程度存在相关性,可能对评估肝纤维化程度有潜在价值。
Objective To investigate the correlation between the levels of serum microRNA-101(miR-101),fatty acid-binding protein 4(FABP4)mRNA and fatty acid-binding protein 5(FABP5)mRNA and the degree of liver fibrosis in patients with viral hepatitis B.Methods Ninety-six patients with viral hepatitis B diagnosed in the First Affiliated Hospital of Henan University from March 2020 to March 2021 were selected and included in hepatitis B group.During the same period,94 healthy examined people in this hospital were selected as control group.According to the degree of liver fibrosis,the patients with viral hepatitis B were divided into S 0 stage(40 cases),S_(1) stage(28 cases),S_(2) stage(16 cases)and S_(3) stage(12 cases).The levels of serum miR-101,FABP4 mRNA and FABP5 mRNA were detected by real-time quantitative polymerase chain reaction(qRT-PCR).Pearson method was used to analyze the correlation between serum miR-101 and the levels of FABP4 mRNA and FABP5 mRNA in patients with viral hepatitis B.Spearman method was used to analyze the correlation between the levels of serum miR-101,FABP4 mRNA and FABP5 mRNA and the degree of liver fibrosis in patients with viral hepatitis B.Results The levels of serum FABP4 mRNA and FABP5 mRNA in hepatitis B group were higher than those in control group,and the level of miR-101 in hepatitis B group was lower than that in control group(P<0.05).The levels of serum FABP4 mRNA and FABP5 mRNA increased with the increase of liver fibrosis stages,and the level of miR-101 decreased with the increase of liver fibrosis stages(P<0.05).The level of serum miR-101 was negatively correlated with FABP4 mRNA and FABP5 mRNA in patients with viral hepatitis B(r=-0.524,-0.590,P<0.05).The degree of liver fibrosis in patients with viral hepatitis B was positively correlated with serum FABP4 mRNA and FABP5 mRNA(r=0.654,0.723,P<0.05),and negatively correlated with miR-101(r=-0.861,P<0.05).Conclusion The levels of serum miR-101,FABP4 mRNA and FABP5 mRNA are correlated with the degree of liver fibrosis in patients wi
作者
谭莉霞
董勇
华静
魏书堂
TAN Lixia;DONG Yong;HUA Jing;WEI Shutang(Department of Gastroenterology,the First Affiliated Hospital of Henan University,Kaifeng 475000,China)
出处
《河南医学研究》
CAS
2022年第1期40-44,共5页
Henan Medical Research
基金
2019年度河南省医学科技攻关计划(联合共建)项目(LHGJ20190532)。
