摘要
单锌指蛋白超家族Dof(DNA binding with one finger)转录因子广泛参与植物的各种生命活动。以甘薯泰中6号基因组为参照挖掘得到46个甘薯Dof基因,每个家族成员均具有C2C2-Dof锌指结构,按照其在染色体上的位置,命名为IbDof1~IbDof46。该基因家族可分为4个亚家族(A~D),且不同亚族的基因结构和基序的分布差异显著。基序1和基序2存在于所有亚家族中;基序5和基序9只存在于亚家族A中;基序6、基序7、基序8和基序10只存在亚家族D中。IbDof的进化极为保守,共有12对染色体复制事件和5对串联重复序列事件(IbDof2/IbDof3、IbDof12/IbDof13、IbDof9/IbDof10、IbDof28/IbDof29和IbDof32/IbDof33),分歧时间平均是3552万年前和186万年前,Ka/Ks比值范围从0.07(IbDof12/IbDof13)到0.68(IbDof6/IbDof25),且与甘薯野生近缘种Ipomoea trifida同一染色体上的Dof同源基因对有38对。组织特异性分析表明,IbDof各亚族在各个组织器官中表达强度不同,且亚族内部呈现不同的表达趋势。IbDof启动子序列中包含逆境和激素响应等相关元件,实时荧光定量PCR进一步分析表明,IDof基因受低温、干旱、高盐和H2O2等非生物胁迫诱导表达。比如大部分IbDof受低温调控表达;IbDof10和IbDof14受干旱胁迫诱导表达;IbDof2、IbDof14、IbDof37、IbDof41和IbDof43受高盐诱导表达;而IbDof8、IbDof10、IbDof25和IbDof41则受H2O2诱导表达,这说明IbDof家族成员协同控制了甘薯的生长发育及参与非生物胁迫过程。
DNA-binding One Zinc Finger(Dof)transcription factors are widely involved in various life activities of plants.Forty-six IbDof genes from sweetpotato cv.Taizhong 6 with a highly conserved Dof domain structured as a C2C2-type zinc finger were identified and named from IbDof1 to IbDof46 according to their position on the chromosomes.IbDof family could be di-vided into four subgroups(A–D),which shared the similar motifs and gene structures.Motif 1 and Motf 2 occurred in all of the identified IbDofs,Motif 5 and Motif 9 only occurred in subgroup A,and Motif 6-Motif 8 and Motif 10 only occurred in subgroup D.Twelve segment duplicated gene pairs and five tandem duplicated gene pairs of IbDofs(IbDof2/IbDof3,IbDof12/IbDof13,IbDof9/IbDof10,IbDof28/IbDof29,and IbDof32/IbDof33)contributed to the expansion of IbDof family in sweetpotato.The average divergence times of segmental duplication gene pairs and tandem duplicated gene pairs seemed to have emerged 35.22 MYA and 1.86 MYA,and the Ka/Ks ratios of the paralogous IbDofs were range from 0.07(IbDof12/IbDof13)to 0.68(Ib-Dof6/IbDof25).Tirty-eight orthologous IbDof gene pairs between sweetpotato and their wild relative species Ipomoea trifida were involved in duplicated genomic blocks based on synteny analysis.Transcriptome analysis indicated different subgroups expressed specifically in various tissues,and IbDofs in the same subgroup also revealed different expression tends.Various hormones and stresses response element were identified in the promoters of IbDof genes,and qRT-PCR demonstrated specific IbDof genes re-sponded to various environmental stresses,including cold,drought,salt,and H2O2.Most IbDof genes were regulated by cold treatment;IbDof10 and IbDof14 were up-regulated by drought treatment;IbDof-2,-14,-37,-41,-43 were up regulated by high salt stress;and IbDof-8,-10,-25,-41 were up regulated by H2O2 treatment.In summary,our result indicated that IbDof family genes coordinately regulated the growth and development of sweetpotato and been involved in the various abio
作者
靳容
蒋薇
刘明
赵鹏
张强强
李铁鑫
王丹凤
范文静
张爱君
唐忠厚
JIN Rong;JIANG Wei;LIU Ming;ZHAO Peng;ZHANG Qiang-Qiang;LI Tie-Xin;WANG Dan-Feng;FAN Wen-Jing;ZHANG Ai-Jun;TANG Zhong-Hou(Xuzhou Institute of Agricultural Sciences of Xuhuai District of Jiangsu Province/Xuzhou Sweetpotato Research Center of Jiangsu Province/Key Laboratory of Sweetpotato Biology and Genetic Breeding,Ministry of Agriculture and Rural Affairs,Xuzhou 221131,Jiangsu,China;Anhui Agri-cultural University,Hefei 230036,Anhui,China)
出处
《作物学报》
CAS
CSCD
北大核心
2022年第3期608-623,共16页
Acta Agronomica Sinica
基金
国家农业现代产业技术体系建设专项(CARS-11-B-13)
农业农村部薯类作物生物学与遗传育种综合性重点实验开放课题(NYBSL201802)资助。
关键词
甘薯
Dof基因家族
系统进化
表达分析
sweetpotato
Dof gene family
evolution analysis
gene expression profiling
