摘要
目的:建立蔓荆子中蔓荆子黄素含量的测定方法。方法:采用高效液相色谱法,选择Agilent SB C18柱(250 mm×4.6 mm,5μm)为色谱柱,以乙腈和0.1%磷酸溶液为流动相(梯度洗脱),流速1.0 mL/min,检测波长254 nm。结果:蔓荆子黄素的线性关系方程为Y=1.42×10^(4)X-2.172×10^(2),在12.5~200μg/mL范围内的峰面积与进样浓度线性关系良好(R^(2)=0.9997)。精密度、重复性及稳定性试验的相对标准偏差(RSD)分别为0.71%、1.05%、0.96%,平均加样回收率为98.19%,其RSD为1.15%。蔓荆子中蔓荆子黄素的平均含量为0.0554%。结论:建立的高效液相色谱法方法简便、准确、快速,重复性好,专属性好,可应用于蔓荆子中蔓荆子黄素含量测定。
Objective:To establish a method for the determination of vitexin in Vitex trifolia.Methods:Agilent SB C18 column(250 mm×4.6 mm,5μm)was used as the chromatographic column with acetonitrile and 0.1%phosphoric acid as mobile phases(gradient elution)at a flow rate of 1.0 mL/min.The detection wavelength was 254 nm.Results:The linear equation of Vitexin was Y=1.42×10^(4)X-2.172×10^(2).The peak area in the range of 12.5-200μg/mL had a good linear relationship with the injection concentration(R^(2)=0.9997).The relative standard deviations(RSD)of precision,repeatability and stability tests were 0.71%,1.05%and 0.96%,respectively.The average recoveries were 98.19%and RSD was 1.15%.The average content of Vitexin in Vitex trifolia was 0.0554%.Conclusion:The established HPLC method is simple,accurate,rapid,reproducible and specific.It can be applied to the determination of Vitexin in Vitex trifolia.
作者
陈贞明
Chen Zhenming(Fujian Pianzihuang Cosmetics Co.,Ltd.,Fujian 363000,China)
出处
《中国野生植物资源》
CSCD
2021年第12期23-25,共3页
Chinese Wild Plant Resources
