摘要
目的探讨血清miR-221-3p和miR-451a相对表达水平在预测卵巢癌患者3年内出现死亡的诊断效能。方法选取2016年1月至2017年12月在该院就诊、病理诊断为卵巢癌的患者110例(卵巢癌组)作为研究对象。选择同期在该院经病理证实的良性卵巢肿瘤患者75例和女性体检健康者45例分别作为卵巢良性组和健康对照组。采用qRT-PCR法检测各组血清miR-221-3p和miR-451a相对表达水平。观察各组血清miR-221-3p和miR-451a相对表达水平变化,卵巢癌患者血清miR-221-3p和miR-451a相对表达水平在术前、术后的变化,以及其与临床指标和预后的关系。结果卵巢癌组血清miR-221-3p相对表达水平明显高于卵巢良性组和健康对照组,差异均有统计学意义(P<0.05);卵巢癌组术后血清miR-221-3p相对表达水平较术前明显降低,差异有统计学意义(t=11.785,P<0.05)。卵巢癌组血清miR-451a相对表达水平较卵巢良性组和健康对照组明显降低,差异均有统计学意义(P<0.05);卵巢癌组术后血清miR-451a相对表达水平较术前明显升高,差异有统计学意义(t=10.833,P<0.05)。卵巢癌患者血清miR-221-3p相对表达水平与血清miR-451a相对表达水平呈负相关(r=-0.648,P<0.01)。血清miR-221-3p和miR-451a相对表达水平在预测卵巢癌患者3年内出现死亡具有较高的效能,联合检测其灵敏度为87.1%,特异度为91.1%,AUC为0.929,AUC均明显高于miR-221-3p(Z=2.016,P<0.05)和miR-451a(Z=2.543,P<0.05)单项检测。结论血清miR-221-3p和miR-451a相对表达水平在预测卵巢癌患者3年内发生死亡具有较高的效能,联合检测能够明显提高预测效能,为是否对卵巢癌患者采取进一步干预措施提供理论依据。
Objective To explore the diagnostic efficacy of serum miR-221-3p and miR-451a relative expression level in predicting death within 3 years in patients with ovarian cancer.Methods From January 2016 to December 2017,110 patients who were pathologically diagnosed as ovarian cancer in a hospital were selected as the ovarian cancer group.75 cases of benign ovarian tumors and 45 cases of healthy physical examinations were selected in a hospital during the same period,which were respectively benign ovarian group and healthy control group.qRT-PCR was used to detect the relative expression level of serum miR-221-3p and miR-451a in each group.The relative expression levels of serum miR-221-3p and miR-451a were observed in each group,the serum miR-221-3p and miR-451a relative expression levels were also observed in patients with ovarian cancer before and after surgery,and their correlation with clinical indicators and prognosis were analyzed.Results The relative expression level of serum miR-221-3p in ovarian cancer group was significantly higher than that in benign ovarian group and healthy control group,and the difference was statistically significant(P<0.05).The relative expression level of miR-221-3p in the ovarian cancer group after operation was significantly lower than that before operation,and the difference was statistically significant(t=11.785,P<0.05).The relative expression level of miR-451a in serum of ovarian cancer group was significantly lower than that of benign ovarian group and healthy control group,and the difference was statistically significant(P<0.05).The relative expression level of miR-451a in the ovarian cancer group was significantly increased after surgery compared with that before surgery,and the difference was statistically significant(t=10.833,P<0.05).The rela-tive expression level of serum miR-221-3p in patients with ovarian cancer was negatively correlated with the relative expression level of serum miR-451a(r=-0.648,P<0.01).The relative expression level of serum miR-221-3p and miR-451a had
作者
裴培
郭春莲
高丹凤
PEI Pei;GUO Chunlian;GAO Danfeng(Department of Obstetrics and Gynecology,Shanghai Tongren Hospital,Shanghai 200050,China)
出处
《检验医学与临床》
CAS
2022年第1期63-67,共5页
Laboratory Medicine and Clinic
