摘要
目的研讨宏基因组学第二代测序在慢性子宫内膜炎(CE)患者微生物检测中的应用及后续对治愈率的影响。方法选取2017年1月至2020年12月在该院生殖中心诊断为CE的不孕不育患者共139例作为研究对象,随机分为两组进行子宫内膜标本微生物培养和宏基因组学第二代测序检测,分析两组的微生物阳性率和CE治愈率。结果组1微生物检测阳性患者17例,阳性率为20.00%;组2微生物检测阳性患者43例,阳性率为79.63%,差异有统计学意义(P<0.05);两组进行相应的抗菌药物治疗后,组2的治愈率显著高于组1,差异有统计学意义(P<0.05)。结论利用宏基因组二代测序的方法,能显著提高患者子宫内膜微生物检测的阳性率和CE患者的治愈率。
Objective To investigate the effect of metagenomics next generation sequencing on the positive rate of microorganism and cure rate in patients with chronic endometritis(CE).Methods A total of 139 infertility patients diagnosed with CE in the reproductive center of a hospital from January 2017 to December 2020 were selected as the research subjects.And randomly divided into two groups for microbial culture and metagenomics next generation sequencing of endometrium specimens.Then the positive rate of microorganism and CE cure rate of the two groups were analyzed.Results In group 1,17 patients were positive for microorganism, the positive rate was 20.00%.In group 2,43 patients were positive for microorganism, the positive rate was 79.63%,and the difference was statistically significant(P<0.05).After the two groups were treated with antibiotics, the cure rate of group 2 was significantly higher than that of group 1,with statistical significance(P<0.05).Conclusion Metagenomics next generation sequencing can significantly improve the positive rate of microorganisms and the cure rate of CE patients.
作者
黄高波
彭芳
李诚
龚燕飞
HUANG Gaobo;PENG Fang;LI Cheng;GONG Yanfei(Reproductive Center,Yueyang Central Hospital,Yueyang,Hunan 414500,China)
出处
《检验医学与临床》
CAS
2022年第1期36-39,共4页
Laboratory Medicine and Clinic
基金
岳阳市科技发展计划专项资金项目(2069999)。
关键词
慢性子宫内膜炎
宏基因组学第二代测序
治愈率
微生物检验
chronic endometritis
metagenomics next generation sequencing
cure rate
microbiological testing
