摘要
试验旨在研究低蛋白日粮补饲蛋氨酸(Met)、赖氨酸(Lys)、亮氨酸(Leu)和异亮氨酸(Ile)对大鼠生长性能、消化性能、血液生化指标及调控肌肉合成信号通路蛋白质表达的影响。选取60只28日龄健康SD大鼠,初始体重为(90.0±1.6) g,随机分为5组,每组12个重复。高蛋白日粮为正对照组(HP组),低蛋白日粮为负对照组(LP组),低蛋白日粮+Met+Lys (LP+ML组),低蛋白日粮+Ile和Leu (LP+IL组),低蛋白日粮+Met、Lys、Ile和Leu (LP+MIIL组)。试验期14 d。结果显示,与HP组相比,其他组大鼠末重、平均日增重均显著降低(P<0.05),肝脏、后肢、肾脏、胃、十二指肠、脾脏、心脏和胴体重等屠宰指标均极显著降低(P<0.01),白蛋白、尿素和甘油三酯含量均显著降低(P<0.05);LP+MIIL组大鼠的IGF-1含量显著高于HP组和LP组(P<0.05);LP+MIIL组和LP+IL组大鼠的生长激素、胰岛素含量显著高于HP组和LP组(P<0.05);HP组和LP组大长鼠的胰高血糖素含量显著高于其他3组(P<0.05);HP组大鼠的色氨酸、苯丙氨酸、亮氨酸、异亮氨酸、缬氨酸、蛋氨酸、酪氨酸和苏氨酸的含量均极显著高于其他组(P<0.01),谷氨酸、天冬酰胺的含量显著高于其他组(P<0.05);LP+ML组、LP+IL组和LP+MIIL组大鼠肌肉组织西罗莫司靶蛋白磷酸化水平显著提高(P<0.05)。研究表明,低蛋白日粮添加赖氨酸、蛋氨酸、亮氨酸和异亮氨酸,提高了大鼠肌肉组织西罗莫司靶蛋白及其下游蛋白质的磷酸化水平,促进肌肉组织蛋白质合成;可维持血糖含量稳定,有助于提高大鼠免疫力,降低不良应激。
The experiment was to study the effect of supplementary methionine(Met), lysine(Lys), leucine(Leu) and isoleucine(Ile) in low-protein diets on growth performance, digestion performance, blood biochemical indicators and regulation of protein expression in muscle synthesis signaling pathway of rat. 60 healthy 28-day-old SD rats with an initial weight of(90.0±1.6) g were selected and randomly divided into five groups, with 12 rats in each replicates. The high-protein diet was the positive control group(HP group), the low-protein diet was the negative control group(LP group), low protein diet+Met + Lys was LP + ML group, low protein diet+Ile and Leu was LP + IL group, and low protein diet+Met, Lys, Ile and Leu was LP + MIIL group. The experiment lasted for 21 d. The results showed that compared with the HP group, the final weight and average daily gain of other groups were significantly reduced(P<0.05).The slaughter indexes of liver, hindlimbs, kidneys, stomach, duodenum, spleen, heart and carcass in other groups were extremely reduced(P<0.01). The contents of albumin, urea and triglycerides in other groups were significantly reduced(P<0.05). IGF-1 content in LP + MIIL group was significantly higher than that in HP group and LP group(P<0.05). The growth hormone and insulin content of rats in LP + MIIL group and LP + IL group were significantly higher than those in HP group and LP group(P<0.05). The GLN content of HP group and LP group was significantly higher than other groups(P<0.05). The content of tryptophan, phenylalanine,leucine, isoleucine, valine, methionine, tyrosine and threonine in the HP group were extremely higher than those in other groups(P<0.01). The content of acid and asparagine was significantly higher than other groups (P<0.05). LP + ML group, LP + IL group and LP + MIIL group significantly increased the phosphorylation level of rapamycin target protein in muscle tissue(P<0.05). The experiment indicates that adding lysine, methionine, leucine and isoleucine to low-protein diets can increase the ph
作者
冯蕾
张宇
张子叶
宫晨
刘伟
蒋一男
胡志勇
FENG Lei;ZHANG Yu;ZHANG Zi-ye;GONG Chen;LIU Wei;JIANG Yi-nan;HU Zhi-yong
出处
《饲料研究》
CAS
北大核心
2021年第22期96-102,共7页
Feed Research
基金
国家自然科学基金项目(项目编号:31772624)。
关键词
大鼠
低蛋白日粮
氨基酸
生长性能
肌蛋白
信号通路
rat
low protein diets
amino acid
growth performance
muscle protein
signal path
