摘要
目的:探讨HIST1H2BH在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的表达及临床意义。方法:利用Oncomine数据库和GEPIA数据库,分析HIST1H2BH在不同类型肿瘤、HNSCC中的表达情况,以及与患者临床病理参数的关系和预后价值。采用免疫组织化学染色法分析临床标本中HIST1H2BH的表达,通过cBioPortal数据库分析HIST1H2BH在HNSCC中的变异及与预后的关系;通过String数据库,初步探讨HIST1H2BH的相互作用蛋白及分子机制。采用SPSS 24.0软件包对数据进行统计学分析。结果:Oncomine数据库和GEPIA数据库分析显示,HIST1H2BH在全身多种肿瘤中呈高表达,在HNSCC中显著高表达,且其表达水平与HNSCC患者的病理分期和预后密切相关,HIST1H2BH高表达的患者,预后较差。临床标本的免疫组织化学染色结果显示,HIST1H2BH在HNSCC患者病灶组织中的表达显著高于癌旁组织。cBioPortal数据库分析显示,HIST1H2BH在HNSCC患者中变异率不高,但与其无病生存率有一定关联。String数据库分析显示,与HIST1H2BH相关联的基因较多,其参与的生物学过程的信号通路也较复杂。结论:HIST1H2BH在HNSCC组织中的表达高于正常组织,其高表达与患者不良预后相关,可作为HNSCC诊断与预后的候选指标。
PURPOSE:The purpose of this study was to investigate the expression of HIST1H2BH in head and neck squamous cell carcinoma(HNSCC)and to analyze its clinical significance.METHODS:The expression of HIST1H2BH in different tumors and HNSCC were analyzed based on the Oncomine and GEPIA database,and its relationship with clinicopathological parameters and prognostic value of HNSCC patients were also analyzed.The expression of HIST1H2BH in clinical specimens were detected by immunohistochemical staining.The variation of HIST1H2BH in HNSCC and its relationship with prognosis were analyzed based on the cBioPortal database.The interaction proteins and molecular mechanism of HIST1H2BH were preliminarily predicted using the String database.SPSS 24.0 software package was used for statistical analysis of the data.RESULTS:Analysis of Oncomine database and GEPIA database showed that HIST1H2BH was highly expressed in variety of tumors,and was significantly highly expressed in HNSCC.The expression level of HIST1H2BH was closely related to the pathological stage and prognosis of HNSCC patients,the prognosis of patients with high HIST1H2BH expression was poor.Immunohistochemical results of clinical specimens showed that the expression of HIST1H2BH in the cancerous tissue of HNSCC patients was significantly higher than that in the adjacent tissues.cBio-Portal database analysis showed that the variation rate of HIST1H2BH in HNSCC patients was not high,but it was related to the disease-free survival rate.String database analysis showed that there were many genes associated with HIST1H2BH,and the signal pathways involved in biological processes were also complex.CONCLUSIONS:The expression of HIST1H2BH is elevated in HNSCC tissues,and its high expression is associated with poor prognosis.HIST1H2BH could be served as a marker for diagnosis and prognosis of HNSCC.
作者
王守鹏
丁紫雪
孟箭
李梦
李晓东
袁壮
WANG Shou-peng;DING Zi-xue;MENG Jian;LI Meng;LI Xiao-dong;YUAN Zhuang(School of Stomatology,Weifang Medical University.Weifang 261053,Shandong Province;Department of Stomatology,Central Hospital of Xuzhou City.Xuzhou 221009,Jiangsu Province;School of Stomatology,Xuzhou Medical University.Xuzhou 221004,Jiangsu Province,China)
出处
《上海口腔医学》
CAS
北大核心
2021年第6期599-605,共7页
Shanghai Journal of Stomatology
基金
国家口腔疾病临床医学研究中心开放课题(NCRCO-202101)
徐州市重点研发计划(KC21187)。
