摘要
目的研究Moesin在非小细胞肺癌中的作用及其机制。方法通过数据库分析发现Moesin高表达在非小细胞肺癌患者中与不良预后相关。利用Western Blot检测Moesin在肺癌细胞株内表达情况。选取Moesin表达量中等A549细胞株进行实验。将A549细胞分为对照组和Moesin干扰组,分别利用CCK-8,Transwell检测细胞增殖、转移的作用,利用Western blot检测干扰Moesin对细胞增殖、上皮间充质转移(EMT)信号通路蛋白的影响。结果干扰Moesin后,增殖信号通路p-AKT,p-ERK,EMT信号通路E-cadherin,N-cadherin,Vimtein等蛋白都有明显变化,并且肺癌细胞表型如增殖,转移、侵袭能力也有明显下降。结论Moesin在非小细胞肺癌中表达上调,其高表达与不良预后相关。并且,通过影响下游增殖、转移信号通路蛋白,Moesin可促进肺癌细胞增殖转移。
Objective To investigate the role and mechanism of Moesin in non-small cell lung cancer(NSCLC).Methods High expression of Moesin was found to be associated with poor prognosis in patients with NSCLC by database analysis.The expression of Moesin in lung cancer cell lines was detected by Western blot.A549 cell lines with moderate Moesin expression were selected for the experiment.A549 cells were divided into the control group and Moesin interference group.CCK-8 and Transwell were used to assess cell proliferation and metastasis.Western blot was used to detect the effects of Moesin interference on cell proliferation and epithelial-mesenchymal transformation(EMT)signal pathway proteins.Results After the interference of Moesin,the proliferation signal pathway p-AKT,p-ERK,EMT signal pathway E-cadherin,N-cadherin,Vimentin were significantly changed,and the phenotype of lung cancer cells such as proliferation,metastasis,and invasion were all significantly inhibited.Conclusion Moesin is up-regulated in NSCLC and correlates with poor prognosis.Moreover,Moesin can promote lung cancer cells proliferation and metastasis via downstream signal pathway proteins.
作者
张若琛
陈义坤
祝清清
张伟杰
黄建安
ZHANG Ruo-chen;CHEN Yi-kun;ZHU Qing-qing;ZHANG Wei-jie;HUANG Jian-an(Department of Respiratory and Critical Care Medicine,First Affiliated Hospital of Soochow University,Institute of Respiratory Diseases of Soochow University,Suzhou,Jiangsu 215000,China)
出处
《临床肺科杂志》
2022年第1期98-101,118,共5页
Journal of Clinical Pulmonary Medicine
基金
国家自然科学基金青年基金(No.81802295)
江苏省自然科学基金青年基金:(No.BK20180198)
苏州呼吸内科重点实验室(No.SZS201617)。
关键词
MOESIN
非小细胞肺癌
增殖
转移
Moesin
Non-small cell lung cancer
Proliferation
Metastasis
