摘要
目的观察"内关"穴浅深层次及相应层次上电针不同频率对抗家兔心律失常的影响。方法将家兔随机分为空白组、模型组、浅深层组(分别为2、20、100 Hz),共8组,每组6只。采用耳缘静脉快速推注4 mg/kg氯化钡建立心律失常模型。治疗组在造模成功后,用1寸绝缘针于家兔"内关"穴处进针,浅层进针3 mm,深层进针5~8 mm,连接电针仪,频率分别为2、20、100 Hz,连续波,各20 min;BL420-N连续监测Ⅱ导联心电图,记录各组心律失常持续时间并观察心肌结构变化以及Na^(+)-K^(+)-ATP酶活性的改变。结果与空白组比较,模型组心律失常发生率为100%;与模型组比较,各治疗组除2 Hz浅层外心律失常持续时间均有缩短(P<0.05)。各组之间,20 Hz深层组<2 Hz深层组<100 Hz深层组<20 Hz浅层组<100 Hz浅层组<2 Hz浅层组。模型组镜下观察心肌纤维排列紊乱、闰盘增厚、可见大量空泡;各治疗组均有不同程度的改善。与空白组相比,模型组Na^(+)-K^(+)-ATP酶值下降明显(P<0.05);除2 Hz和100 Hz浅层组外,各治疗组均有提高。结论内关穴浅深层次均有对抗心律失常的作用,但各层次上存在适宜的刺激频率;只有适宜的层次采用适宜的刺激频率才能发挥穴位的最佳效应,其中20 Hz深层针刺效应最佳。
Objective:To investigate the difference of stimulation effect of electroacupuncture at shallow and deep levels of Neiguan acupoint for rabbits with arrhythmia.Methods:Rabbits were randomly divided into a blank group,a model group,and a shallow-deep layer group(respectively 2,20,100 Hz),a total of 8 groups,each with 6 rabbits.The arrhythmia model was established by rapid injection of 4 mg/kg barium chloride through the ear vein.In the treatment group,after successful model establishment,a 1-inch insulated needle was used to insert a needle at the Neiguan acupoint of the rabbit.The shallow needle was inserted 3 mm,and the deep needle was inserted 5~8 mm.The electric acupuncture instrument was connected with the frequency of 2,20,100 Hz,continuous wave,20 min each;BL420-N continuously monitored theⅡlead electrocardiogram,recorded the duration of arrhythmia in each group,and observed the changes of myocardial structure and the changes of Na^(+)-K^(+)-ATPase activity.Results:Compared with the blank group,the incidence of arrhythmia in the model group was 100%;compared with the model group,the duration of arrhythmia in each treatment group was shortened except for the 2 Hz superficial layer(P<0.05).Among each group,the 20 Hz deep layer group<2 Hz deep group<100 Hz deep group<20 Hz shallow group<100 Hz shallow group<2 Hz shallow group.In the model group,the myocardial fiber arrangement was disordered,the intercalary disc thickened,and a large number of vacuoles were observed under the microscope;each treatment group had different degrees of improvement.Compared with the blank group,the Na^(+)-K^(+)-ATPase value of the model group decreased significantly(P<0.05);except for the 2 Hz and 100 Hz shallow groups,all treatment groups increased.Conclusion:Neiguan acupoint has the effect of antagonizing arrhythmia at the shallow and deep levels,but there are appropriate stimulation frequencies at all levels;only the appropriatestimulation frequency at the appropriate level can exert the best effect of the acupuncture points,of
作者
杨守亲
古春凌
张硕峰
林怡
彭超
吴晓玲
徐畅
孙千惠
杨智文
高小峰
程凯
Yang Shouqin;Gu Chunling;Zhang Shuofeng;Lin Yi;Peng Chao;Wu Xiaoling;Xu Chang;Sun Qianhui;Yang Zhiwen;Gao Xiaofeng;Cheng Kai(Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国中医急症》
2021年第12期2094-2098,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
国家虚拟仿真实验教学项目(XNFZ18002)
横向课题北京麦迪克斯科技有限公司(2030071720012)。
关键词
快速性心律失常
电针
内关穴
层次
针刺深度
兔
Tachyarrhythmia
Electroacupuneture
Neiguan acupoint
Level
Acupuncture depth
Rabbits
