摘要
目的确证蛹虫草多糖(CMPS)对高脂饮食诱导的ApoE^(-/-)小鼠非酒精性脂肪性肝病(NAFLD)的保护作用,并探讨相关机制。方法将32只ApoE^(-/-)小鼠随机分为ApoE^(-/-)组及CMPS(25、50、100μg/g)组,均高脂饲养,CMPS组同时喂饲相应剂量CMPS干预。另取8只C57BL/6小鼠普通饲料喂饲作为野生对照组(WT组)。12周后取材,计算小鼠肝脏指数,HE染色观察肝脏组织学改变,按照试剂盒说明检测血清甘油三酯(TG)浓度及肝脏TG浓度、超氧化物歧化酶(SOD)活性、过氧化脂质(LPO)含量。荧光定量PCR及Western blot分析肝组织中过氧化物酶体增殖物激活受体α(PPARα)及酰基辅酶A氧化酶1(ACOX1)的表达水平。结果与ApoE^(-/-)组相比,CMPS干预可明显降低小鼠肝脏质量和肝脏指数,减轻高脂饮食诱导的肝脂肪变性,逆转肝脏、血清中TG水平的升高。同时,CMPS组肝脏中PPARα及ACOX1的mRNA和蛋白表达均上调。此外,CMPS喂饲可增加ApoE^(-/-)小鼠肝脏中抗氧化酶SOD活性,降低肝脏LPO含量。结论CMPS有效改善高脂饮食诱导的ApoE^(-/-)小鼠非酒精性脂肪性肝病,其机制可能与其促进肝内脂肪酸氧化和抑制氧化应激有关。
Aim To confirm the protective effect of cordycepsmilitaris polysaccharide(CMPS)on nonalcoholic fatty liver disease(NAFLD)in ApoE^(-/-) mice fed with high-fat diet and explore the underlying mechanism.Methods 32 ApoE^(-/-) mice were randomly divided into ApoE^(-/-) group and CMPS(25,50,100μg/g)treatment groups,all of which were fed with high-fat diet.The treatment groups were simultaneously fed with respective doses of CMPS.Another 8 C57BL/6 mice were fed with chow diet as control group(WT group).After 12-weeks experimental period,the mice were sacrificed.The liver index of mice was calculated and the histological changes of liver were observed by HE staining.The serum triglyceride(TG)concentration,liver TG concentration,superoxide dismutase(SOD)activity and lipid peroxide(LPO)content were detected according to the kit instructions.The levels of peroxisome proliferator-activated receptorα(PPARα)and acyl-CoA oxidase 1(ACOX1)were analyzed by quantitative PCR and Western blot.Results Compared with ApoE^(-/-) group,CMPS treatment significantly reduced liver weight and liver index,alleviated hepatic steatosis induced by high-fat diet,and reversed the increasement of TG levels in liver and serum.Meanwhile,the mRNA and protein expressions of PPARαand ACOX1 were up-regulated in liver of CMPS treatment groups compared with those of ApoE^(-/-) group.In addition,CMPS treatment enhanced the activity of SOD and lowered the content of LPO in the liver of ApoE^(-/-) mice.Conclusion CMPS can effectively improve NAFLD induced by high-fat diet in ApoE^(-/-) mice,which may be related to its promotion of fatty acid oxidation and inhibition of oxidative stress.
作者
陈刘欣
于凤秀
朱文君
贾修滨
李宝华
赵上
张颖
田华
司艳红
秦树存
CHEN Liuxin;YU Fengxiu;ZHU Wenjun;JIA Xiubin;LI Baohua;ZHAO Shang;ZHANG Ying;TIAN Hua;SI Yanhong;QIN Shucun(Shandong First Medical University&Shandong Academy of Medical Sciences,Tai an,Shandong 271000,China;Shandong University of Traditional Chinese Medicine,Ji nan,Shandong 250355,China)
出处
《中国动脉硬化杂志》
CAS
2022年第3期219-224,共6页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金资助项目(81600681)
山东第一医科大学学术提升计划(2019QL010、2019PT009)
国家级大学生创新创业训练计划项目(201910439038)
山东省重点研发计划项目(2019gsf108260)。
关键词
蛹虫草多糖
非酒精性脂肪性肝病
甘油三酯
氧化应激
cordycepsmilitaris polysaccharides
nonalcoholic fatty liver disease
triglyceride
oxidative stress
