摘要
从罹病的马唐(Digitaria sanguinalis)植株上分离得到1株病原真菌NBERC_H56,鉴定为Alternaria perpunctulata,其发酵液对马唐幼苗的生长具有明显的抑制作用。为明确菌株NBERC_H56的活性物质基础,对其次级代谢产物进行研究,通过菌株大量发酵,经提取和制备高效液相色谱分离纯化得到3个化合物,经LC-MS以及NMR鉴定为Curvulin(1)、Alternariol(2)和Zinniol(3)。研究首次发现真菌Alternaria perpunctulata具有除草活性,并首次对该菌产生的次级代谢产物进行了分离鉴定。
A pathogenic fungus NBERC_H56 was isolated from the diseased plant of Digitaria sanguinalis,identified as Alternaria perpunctulata,and its fermentation broth had an obvious inhibitory effect on the growth of the seedlings of Digitaria sanguinalis.In or⁃der to clarify the active substance basis of NBERC_H56,the secondary metabolites of the strain were studied.The results showed that three compounds were obtained by large-scale fermentation,extraction and preparation via high performance liquid chromatography,and were identified as Curvulin(1),Alternariol(2)and Zinniol(3)by LC-MS and NMR.Alternaria perpunctulata was found to have herbicidal activity for the first time,and its secondary metabolites were isolated and identified for the first time.
作者
吴兆圆
任梦瑶
张志刚
刘芳
张亚妮
方伟
WU Zhao-yuan;REN Meng-yao;ZHANG Zhi-gang;LIU Fang;ZHANG Ya-ni;FANG Wei(Hubei Biopesticide Engineering Research Centre,Wuhan 430064,China;College of Medical,Wuhan University of Science and Technology,Wuhan 430081,China)
出处
《湖北农业科学》
2021年第24期98-100,129,共4页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心项目(2019-620-000-001-27)。
