摘要
目的:检测miR-142-3p和S期激酶相关蛋白2(SKP2)在老年结直肠癌患者肿瘤组织中的表达,探讨二者的关系及临床意义。方法:选择2015年1月至2016年11月在本院就诊并手术治疗的老年结直肠癌患者的肿瘤组织标本60份作为结直肠癌组,相应癌旁组织标本作为对照组。采用qRT-PCR法检测结直肠癌组织与癌旁组织中miR-142-3p和SKP2 mRNA表达水平,免疫组织化学染色检测SKP2蛋白表达水平,Pearson法分析miR-142-3p和SKP2表达的相关性,分析二者与结直肠癌临床病理特征及预后的关系。结果:miR-142-3p在结直肠癌组织中表达水平低于正常结直肠黏膜组织(P<0.05),SKP2在结直肠癌组织中表达水平高于癌旁组织(P<0.05);miR-142-3p和SKP2表达水平与结直肠癌分化程度及淋巴转移有关(P<0.05);miR-142-3p高表达组患者3年生存率显著高于miR-142-3p低表达组,SKP2高表达组患者3年生存率显著低于SKP2低表达组(P<0.05);淋巴结转移、分化程度低、miR-142-3p低表达、SKP2高表达是结直肠癌患者预后不良的独立危险因素(P<0.05);结肠癌组织中miR-142-3p与SKP2 mRNA表达水平呈负相关(P<0.05)。结论:老年结直肠癌患者肿瘤组织中miR-142-3p低表达、SKP2高表达,且二者与患者生存质量有关,可能成为评价结直肠癌预后的标志物。
Objective:To detect the expression of miR-142-3p and S-phase kinase-associated protein 2(SKP2)in colorectal cancer tissues of the elderly,and to explore the expression relationship and clinical significance of the two.Methods:From January 2015 to November 2016,Sixty tumor tissue specimens of elderly patients with colorectal cancer who were treated and treated in our hospital were selected as the colorectal cancer group,and the corresponding para-cancerous tissue specimens were selected as the control group.The expressions of miR-142-3p and SKP2 mRNA in colorectal cancer tissues and para-cancerous tissue were detected by qRT-PCR,the expression of SKP2 protein was detected by immunohistochemistry,the correlation between the expressions of miR-142-3p and SKP2 was analyzed by Pearson method,and the correlations between them and clinicopathological characteristics,prognosis of colorectal cancer were analyzed.Results:The expression level of miR-142-3p in colorectal cancer tissues was lower than that in para-cancerous tissue(P<0.05),the expression level of SKP2 in colorectal cancer tissues was higher than that in normal colorectal mucosa tissues(P<0.05);the expression levels of miR-142-3p and SKP2 were related to the differentiation degree and lymph metastasis of colorectal cancer(P<0.05);the 3-year survival rate of patients with high expression of miR-142-3p was significantly higher than that of patients with low expression of miR-142-3p,and the 3-year survival rate of patients with high expression of SKP2 was significantly lower than that of patients with low expression of SKP2(P<0.05);lymph node metastasis,low differentiation,low expression of miR-142-3p and high expression of SKP2 were independent risk factors for poor prognosis of colorectal cancer(P<0.05);and there was a negative correlation between miR-142-3p and SKP2 mRNA expression levels in colon cancer(P<0.05).Conclusion:The expression of miR-142-3p is low in elderly colorectal cancer,and the expression of SKP2 is high,they are related to the quality of life
作者
张静
赵振伟
郭学海
王海娟
杨瑞英
ZHANG Jing;ZHAO Zhen-wei;GUO Xue-hai;WANG Hai-juan;YANG Rui-ying(Department of General Surgery,Linxi Hospital,Kailuan General Hospital,Tangshan063103,China)
出处
《中国现代普通外科进展》
CAS
2021年第12期953-957,963,共6页
Chinese Journal of Current Advances in General Surgery
基金
河北省2020年度医学科学研究课题计划项目(20201298)。
