摘要
目的:分析核仁小分子RNA(snoRNA)SNORA51在原发性肝细胞癌组织中的表达情况及临床意义。方法:选取我院2015年1月1日至2017年1月1日诊断的158例原发性肝细胞癌患者为研究对象,采用实时定量聚合酶链反应(qRT-PCR)分析癌组织和癌旁组织中SNORA51的表达情况,运用单因素和多因素COX回归分析其对预后的影响。结果:qRT-PCR检测结果显示,SNORA51在肝癌组织中的表达明显高于癌旁组织(t=21.327,P<0.05),并且配对差异分析同样显示肝癌组织和癌旁组织SNORA51表达差异有统计学意义(t=15.358,P<0.05)。starBase 3.0网站分析显示同样结果(t=2.79,P=0.024)。SNORA51表达的高低与门脉癌栓、血管侵犯和TNM相关,而与性别、年龄、肿瘤大小、HBsAg、AFP、肝硬化无关。高表达组患者的中位生存时间为15个月(95%CI:12.539~17.461),低表达组患者的中位生存时间为37个月(95%CI:27.472~46.528),两组患者预后差异有统计学意义(Log-rankχ^(2)=16.077,P<0.05),提示SNORA51表达与预后呈负相关。单因素和多因素COX回归分析结果亦显示SNORA51是影响肝癌患者预后的独立危险因素。Uclcan数据库对SNORA51甲基化分析分析结果显示,肝癌中SNORA51启动子区域的甲基化明显降低。结论:SNORA51在肝癌发生中发挥致癌作用,且其高表达与预后不良明显相关,是预后独立危险因素,致癌机制可能与肝癌SNORA51启动子区域高甲基化有关。
Objective:To analyze the expression and clinical significance of nucleolar small molecule RNA(snoRNA)SNORA5 in primary hepatocellular carcinoma.Methods:158 patients with primary hepatocellular carcinoma(liver cancer)diagnosed in our hospital from January 1,2015 to January 1,2017 were selected as the research object.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to analyze the expression of SNORA51 in liver cancer and adjacent tissues.Single factor and multi-factor COX regression was used to analyze its impact on prognosis.Results:The results of qRT-PCR showed that the expression of SNORA51 in liver cancer was significantly higher than that in adjacent tissues(t=21.327,P<0.05),and paired difference analysis also showed that the expression of SNORA51 in liver cancer and adjacent tissues was significantly different(t=15.358,P<0.05).In addition,the analysis of the starBase 3.0 website showed the same results(t=2.79,P=0.024).The expression level of SNORA51 is related to portal tumor thrombus,vascular invasion and TNM,but not to gender,age,tumor diameter,HBsAg,AFP and cirrhosis.The median survival time of patients in the high expression group was 15(95%CI:12.539~17.461)months,and the median survival time of patients in the low expression group was 37(95%CI:27.472~46.528)months.The difference was significant(Log-rankχ^(2)=16.077,P<0.05),suggesting that the expression of SNORA51 was significantly negatively correlated with the prognosis.At the same time,single-factor and multi-factor COX regression analysis results also show that SNORA51 is an independent risk factor affecting the prognosis of liver cancer patients.Uclcan database analysis of SNORA51 methylation analysis showed that the methylation of SNORA51 promoter region in liver cancer was significantly reduced.Conclusion:SNORA51 plays a carcinogenic role in hepatocellular carcinoma,and its high expression is significantly associated with poor prognosis and is an independent prognostic risk factor.The carcinogenic mechanism may be related to the
作者
马驰
焦振东
MA Chi;JIAO Zhen-dong(Hepatobiliary,Pancreatic and Splenic Surgery,Baoji People's Hospital of Shaanxi Province,Baoji 721000,China)
出处
《中国现代普通外科进展》
CAS
2021年第12期944-948,共5页
Chinese Journal of Current Advances in General Surgery
