摘要
DNA提取是获取DNA条形码的关键步骤及进行后续相关研究的基础。小型节肢动物因个体微小,常用的基因组DNA提取方法破坏了重要的外部形态特征,致使无法进行后续的形态学验证,而无形态损伤提取DNA的方法有效弥补前者的不足。本文对小型节肢动物的无形态损伤提取基因组DNA进行综述,对比了小型节肢动物不同基因组DNA提取方法的优缺点,为进一步优化改进,探索更简单、高效的无形态损伤性DNA提取方法奠定基础。
DNA extraction represents the key step for DNA barcoding and the basis of subsequent field study.Common genomic DNA extraction technique may result in damage to the important external morphological nature of microarthropods due to their smaller size,which makes it impossible to further identify the morphology.However,a method of DNA extraction without morphological damage can effectively make up for the deficiency of the former.This paper reviewed the genomic DNA extraction technique without morphological damage to the microarthropods,and compared the advantages and disadvantages of different genomic DNA extraction procedures for microarthropods,with an attempt to lay a foundation for further optimization,improvement and exploration of a simpler and more efficient DNA extraction technique without morphological damage.
作者
范海页
陈慧林
朱永航
王严
王若航
赵薇
陶香林
孙恩涛
FAN Hai-ye;CHEN Hui-lin;ZHU Yong-hang;WANG Yan;WANG Ruo-hang;ZHAO Wei;TAO Xiang-lin;SUN En-tao(Medical Laboratory Science,Wannan Medical College,Wuhu 241002,Anhui Province,China)
出处
《热带病与寄生虫学》
CAS
2021年第6期348-350,357,共4页
Journal of Tropical Diseases and Parasitology
基金
国家自然科学基金项目(31870352)
皖南医学院2019年青年卓越人才资助项目(WYQNYX201902)
安徽省级大学生创新创业项目(S202010368034)。
