摘要
目的:研究大苞雪莲石油醚部位(PESI)及其活性单体成分二十八烷对高原缺氧小鼠大脑和心肌组织的保护作用。方法:将50只BALB/c雄性小鼠随机分为健康对照组、模型对照组、乙酰唑胺组(乙酰唑胺200 mg/kg)、PESI组(PESI200 mg/kg)和二十八烷组(二十八烷100 mg/kg),每组10只,单次尾静脉注射给药。采用低压低氧动物实验舱模拟海拔6000 m高原环境,减压缺氧8 h。HE染色观察高原缺氧小鼠心肌和脑组织显微结构的变化;采用WST-1法、ABTS法、硫代巴比妥酸法检测小鼠血浆、心肌和脑组织中超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)和丙二醛含量;采用比色法和微板法检测小鼠血浆、心肌和脑组织中乳酸含量和乳酸脱氢酶(LDH)活性;采用比色法检测小鼠心肌和脑组织中ATP含量和ATP酶活性。结果:PESI和二十八烷均能减轻高原缺氧小鼠心肌和脑组织的病理损伤;增强血浆、心肌和脑组织中SOD活性、T-AOC、LDH活性,降低丙二醛、乳酸含量;增加心肌和脑组织中ATP含量;同时增强心肌组织钠钾ATP酶、镁ATP酶、钙ATP酶、钙镁ATP酶活性,增强脑组织镁ATP酶、钙镁ATP酶活性。结论:PESI及二十八烷均能减轻高原缺氧小鼠心肌和脑组织损伤,提高机体抗氧化能力,减少自由基损伤,促进细胞无氧酵解,缓解缺氧导致的能量缺乏和代谢障碍。
Objective:To investigate the active compounds from Saussurea involucrata on the heart and brain of mice at simulated high altitude.Methods:Fifty healthy male adult BALB/c mice were randomly divided into normal control group,hypoxic model group,acetazolamide group,petroleum ether extract of Saussurea involucrata(PESI)group and octacosan group with 10 mice in each group.Acetazolamide group,PESI group and octacosan group were treated with acetazolamide(200 mg/kg),PESI(200 mg/kg)or octacosan(100 mg/kg)by single tail vein injection,respectively.Except normal control group,the mice were exposed to a simulated high altitude of 6000 m for 8 h in an animal decompression chamber.After the mice were sacrificed by cervical dislocation,the heart and brain were histologically observed by HE staining;superoxide dismutase(SOD)activity,total anti-oxidant capacity(T-AOC)and the content of malondialdehyde(MDA)in plasma,heart and brain tissues were detected by WST-1 method,ABTS method and TBA method,respectively;lactic acid and lactate dehydrogenase(LDH)activity in plasma,heart and brain tissues were detected by colorimetric method and microwell plate method,respectively;ATP content and ATPase activity in heart and brain tissues were detected by colorimetric method.Results:PESI and octacosane significantly attenuated the pathological damages of heart and brain tissue at simulated high altitude;increased SOD activity,T-AOC and LDH activity,and decreased the contents of MDA and lactic acid in plasma,heart and brain tissues;increased the content of ATP in heart and brain tissues;increased the activities of Na^(+)-K^(+)ATPase,Mg^(2+)ATPase,Ca^(2+)ATPase and Ca^(2+)-Mg^(2+)ATPase in myocardial tissue;and increased the activities of Mg2+ATPase,Ca^(2+)-Mg^(2+)ATPase in brain tissue.Conclusion:PESI and octacosan exert anti-hypoxic activity by improving the antioxidant capacity,reducing the free radical levels,promoting the anaerobic fermentation,and alleviating the energy deficiency and metabolic disorders caused by hypoxia in mice.
作者
邹蓓蕾
田贻婷
石志群
张汝学
马慧萍
ZOU Beilei;TIAN Yiting;SHI Zhiqun;ZHANG Ruxue;MA Huiping(College of Pharmacy,Lanzhou University,Lanzhou 730000,China;Key Laboratory of the Plateau Medicine,the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army,Lanzhou 730050,China)
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2021年第5期568-574,共7页
Journal of Zhejiang University(Medical Sciences)
基金
国家自然科学基金(81571847,81872796)
军队后勤科研计划(CWH17J010,CLB18J028)。
关键词
高原病
缺氧
大苞雪莲石油醚部位
二十八烷
自由基代谢
能量代谢
小鼠
Altitude sickness
Hypoxia
Petroleum ether extract ofSaussurea involucrata
Octacosan
Free radical metabolism
Energy metabolism
Mice
