摘要
目的通过分子探针^(99)Tc^(m)-3PRGD_(2) SPECT在体观察药物注射于大鼠不同部位后显像剂摄取变化,探究不同注射部位对胶原诱导型类风湿性关节炎大鼠模型的影响,建立在体评估的类风湿关节炎大鼠模型评估体系。方法取20只雌性SD大鼠(6~7周龄),随机抽样法分为实验组(A组、B组、C组)及对照组。实验组注射牛II型胶原乳剂(1.0 ml/只,1 g/L),A组注射于鼠尾根部,B组注射于背部皮下,C组注射于腹腔。1周后剂量减半,相同部位再次免疫注射。以两次皮下注射生理盐水为对照组。4周后进行视觉评估、^(99)Tc^(m)-3PRGD_(2) SPECT显像及病理学分析,对比统计分析。结果CIA(胶原诱导性关节炎)组成功造模7只(A组3只,B组2只,C组2只),成模率为47%。CIA各组T/NT比值差异无统计学意义(P>0.05),与空白对照组比较有统计学意义(F=25.668,P<0.05)。CIA各组关节病理滑膜细胞增生指数也明显高于空白对照组(H=9.218,P<0.05)。结论3种不同注射方式均可建立CIA大鼠模型,但背部及腹腔注射可能造成明显炎症反应,且^(99)Tc^(m)-3PRGD_(2)有助于为CIA模型的构建提供活体可视化依据。
Objective To explore the effects of different injection parts on collagen-induced rheumatoid arthritis(CIA)rats by^(99)Tc^(m)-3PRGD_(2) SPECT and establish an in vivo evaluation system for rheumatoid arthritis rat model.Methods A total of 20 female SD rats(age:6~7 weeks)were divided into CIA groups A(n=5),group B(n=5),group C(n=5)and blank control group(n=5)by simple random sampling method.The three CIA groups were injected with bovine type II collagen emulsion(1.0 ml/head,1 g/L),group A was injected into the root of rat tail,group B was injected subcutaneously on the back,and group C was injected into the abdominal cavity.Four weeks later,^(99)Tc^(m)-3PRGD_(2) SPECT imaging,pathological analysis,and comparative statistical analysis were performed.Results The CIA group successfully made 7 models(n group A=3,n group B=2,n group C=2),and the molding rate was about 47%.There was no statistically significant difference in the T/NT ratio between the CIA groups(P>0.05),which was statistically significant compared with the blank control group(F=25.668,P<0.05).The proliferation index of joint pathological synovial cells in each group of CIA was also significantly higher than that of the blank control group(H=9.218,P<0.05).Conclusion Three different injection methods can establish a CIA rat model,but back and abdominal injection may cause obvious inflammation.And^(99)Tc^(m)-3PRGD_(2) could contribute to the construction of CIA model to provide in vivo visualization basis.
作者
曲宏
王国玉
田新月
孙浩
王宏宇
王城
肖瑞
QU Hong;WANG Guo-yu;TIAN Xin-yue;SUN Hao;WANG Hong-yu;WANG Cheng;XIAO Rui(Department of Nuclear Medicine,the Affiliated Hospital of Inner Mongolia Medical University,Key Laboratory of Molecular Imaging,Inner Mongolia Autonomous Region,Hohhot 010010 China;Department of Otorhinolaryngology,People's Hospital of Inner Mongolia Autonomous Region,Hohhot 010010 China;Key Laboratory of Molecular Pathology,Inner Mongolia Medical University,Hohhot 010050 China)
出处
《内蒙古医学杂志》
2021年第12期1417-1419,F0004,共4页
Inner Mongolia Medical Journal
基金
自治区重点实验室开放项目(编号:NYFZBL202103)。
关键词
关节炎
类风湿
放射性核素显像
精氨酸-甘氨酸-天冬氨酸
大鼠
arthritis rheumatoid
neovascularization pathologic
radionuclide imaging
arginine-glycine-aspartic acid
Rats
