摘要
背景:骨科内固定物感染中形成的细菌生物膜是临床非常棘手的问题。次氯酸作为一种高效消毒剂,可引起细菌生物膜破坏,可能对骨科内固定物感染治疗有帮助。目的:研究次氯酸溶液体外浸泡不同时间及灌注对骨科内固定物表面细菌生物膜的清除作用。方法:将金黄色葡萄球菌和铜绿假单胞菌分别在骨科钢板内固定物上定植3 d后形成细菌生物膜,扫描电镜下确认细菌生物膜已形成。采用次氯酸溶液分别浸泡已形成两种细菌生物膜的内固定物0,5,10,20 min及20 min+灌注6 L次氯酸和生理盐水的混合溶液(每3 L生理盐水加入120 mL次氯酸),扫描电镜下观察细菌生物膜形态,评估次氯酸溶液对内固定物细菌生物膜的清除效果。结果与结论:①铜绿假单胞菌:扫描电镜下可见,未经次氯酸溶液浸泡的内固定物上的细菌生物膜斑块面积大且较厚,菌落密集集中;经过次氯酸浸泡5,10,20 min后,细菌生物膜斑块面积逐渐减小并变薄,次氯酸溶液浸泡20 min+灌注6 L次氯酸和生理盐水的混合溶液可清除细菌生物膜;②金黄色葡萄球菌:扫描电镜下的表现与铜绿假单胞菌生物膜一致;③结果表明:延长浸泡时间或联合灌注可增强次氯酸对内固定物细菌生物膜的清除作用;次氯酸溶液浸泡足够时长可降解破坏内固定物上定植的细菌生物膜,清除菌落,从而达到消毒目的。
BACKGROUND:Biofilm formation during the infection of orthopedic fixation devices is a very difficult clinical problem.Hypochlorous acid,as a highly effective disinfectant,can destroy the bacterial biofilm,which may be helpful for treating infection of orthopedic fixation devices.OBJECTIVE:To study the scavenging effect of hypochlorous acid on bacterial biofilm on the surface of orthopedic fixation devices after soaking and perfusion for different time in vitro.METHODS:Staphylococcus aureus and Pseudomonas aeruginosa were colonized on orthopedic steel fixation devices for 3 days,and then the bacterial biofilm formed,which was confirmed by scanning electron microscope.Orthopedic fixation devices with two kinds of bacterial biofilms were soaked in hypochlorous acid for 0,5,10,and 20 minutes,or perfused with 6 L of a mixed solution of hypochlorous acid and saline(every 3 L of saline with addition of 120 mL of hypochlorous acid)for 20 minutes.The morphology of bacterial biofilms was observed under scanning electron microscope,and the scavenging effect on the bacterial biofilm on the surface of orthopedic internal fixation devices was then evaluated.RESULTS AND CONCLUSION:Pseudomonas aeruginosa:Under the scanning electron microscope,the bacterial biofilm plaques on the surface of the internal fixation device that was not soaked in hypochlorous acid solution were large and thick,and the colonies were densely concentrated.After 5,10,and 20 minutes soaking in hypochlorous acid,the bacterial biofilm plaque area was gradually decreased and became thinner.Perfusion with 6 L of a mixture of hypochlorous acid and saline for 20 minutes could remove the bacterial biofilm.Staphylococcus aureus:The performance under the scanning electron microscope was consistent with the biofilm of Pseudomonas aeruginosa.To conclude,prolonging the soaking time or a mixed perfusion can enhance the scavenging effect of hypochlorous acid on the bacterial biofilm on the surface of internal fixation devices.By soaking in hypochlorous acid for a suffic
作者
梁金正
邵欣欣
杜少华
戴双武
Liang Jinzheng;Shao Xinxin;Du Shaohua;Dai Shuangwu(Department of Musculoskeletal Oncology,Center for Orthopedic Surgery,the Third Affiliated Hospital of Southern Medical University,Guangzhou 510630,Guangdong Province,China;Department of Anesthesiology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第15期2367-2371,共5页
Chinese Journal of Tissue Engineering Research
基金
广东省科技计划项目(2020A1414050028),项目负责人:戴双武。
关键词
次氯酸
细菌生物膜
骨科手术
骨科内固定物
感染
消毒剂
hypochlorous acid
bacterial biofilm
orthopedic surgery
orthopedic internal fixation device
infection
disinfectant
