摘要
为挖掘可能参与烟草花序发育和抗逆胁迫相关的花序分生组织类受体激酶(Inflorescence meristem receptor-like kinase,IMK)基因家族成员,利用生物信息学方法在普通烟草基因组中鉴定出6个IMK基因家族成员,不同成员间的氨基酸序列一致性在61%~97%之间。系统进化树分析显示烟草IMKs主要分为2类,主要区别在于重复motif 12(GSIP motif)的个数差异。跨膜结构域分析显示,2种类型烟草IMKs跨膜结构域分布间存在差异,暗示这2类IMKs可能出现了功能分化。翻译后修饰位点分析结果表明,在拟南芥中鉴定到的IMK2的11个修饰位点,其中只有2个位点在所有烟草IMKs蛋白中均保守,推断这2个位点可能在IMKs蛋白功能发挥的过程中起着重要作用。表达分析表明,大部分IMKs在侧根和花蕾中高表达,而且被GA、IAA等激素诱导上调表达,暗示IMKs蛋白可能参与侧根和花的发育过程,同时也参与GA和IAA介导的调控反应。
In order to identify the members of inflorescence meristem receptor-like kinase(IMK)gene family which thought to be involved in tobacco inflorescence development and stress resistance,six IMK gene family members were identified in tobacco genome using bioinformatics methods,and the similarity of amino acid sequences among the different members were between 61%and 97%.Phylogenetic tree analysis showed that tobacco IMKs could mainly be divided into two types,and the number of repeating motif 12(GSIP motif)was the major difference between the two types.Transmembrane domain analysis indicated that there were differences in the distribution of transmembrane domains between the two types of tobacco IMKs,suggesting that there might be functional differentiation between them.The results of post-translational modification site analysis showed that only two of the 11 modification sites identified in Arabidopsis IMK2 were conserved in all IMK proteins,suggesting that these two sites might play important roles in the function of IMK proteins.Gene expression analysis showed that the IMKs were highly expressed in lateral roots and flower buds,and were up-regulated by GA,IAA and other hormones,suggesting that IMK proteins might be involved in the development of lateral roots and flowers and participate in GA and IAA mediated regulatory responses.
作者
陈千思
刘萍萍
李泽锋
杨小宁
武明珠
郑庆霞
周会娜
CHEN Qiansi;LIU Pingping;LI Zefeng;YANG Xiaoning;WU Mingzhu;ZHENG Qingxia;ZHOU Huina(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2021年第12期1-8,共8页
Tobacco Science & Technology
基金
河南省重点研发与推广专项(科技攻关)项目“LRR类受体蛋白激酶NtIMK2在植物干旱胁迫应答过程中的功能机制解析”(192102110003)。
