摘要
目的不同浓度生长因子对细胞增殖有不同的效应,本研究主要寻找促进细胞增殖的最佳血小板衍生生长因子(platelet derived growth factor,PDGF-BB)和血管内皮生长因子(vascular endothelial growth factor,VEGF-A)浓度。方法将慢病毒GFP转染至人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC),并将PDGF-BB和VEGF-A以浓度各进行分组:0μg/mL GFs组(Control组),0.02μg/mL GFs组,0.2μg/mL GFs组,2μg/mL GFs组,5μg/mL GFs组。将GFP-HUVEC接种到孔板或者凝胶中,培养一定时间后利用荧光显微镜或者CCK-8检测其增殖效果。结果 (1)转染了GFP的HUVEC状态良好,镜下可见较强荧光。(2)孔板内辅以不同浓度生长因子培养HUVEC,分别在2μg/mL PDGF-BB(P <0.001)和0.02μg/mL VEGF-A(P <0.000 1)细胞有最高的增殖活性。(3)CCK-8显示在2μg/mL PDGF-BB(P <0.05)和0.02μg/mL VEGF-A(P <0.001)细胞有最高的增殖活性(4)将细胞包裹进水凝胶内并在培养基中给予不同浓度生长因子培养,分别在2μg/mL PDGF-BB(P <0.01)和0.02μg/mL VEGF-A(P <0.01)细胞有最高的增殖活性。结论 2μg/mL PDGF-BB和0.02μg/mL VEGF-A能对细胞产生的增殖效果最好,过高浓度的生长因子对于细胞可能有抑制作用。
Objective The same concentration of growth factor has different effects on cell proliferation and the aim of this study was to find the optimal concentrations of PDGF-BB and VEGF-A for HUVEC proliferation.Methods Lentivirus GFP was transfected into HUVECs and PDGF-BB and VEGF-A were grouped in concentrations:0 μg/mL GFs(Control),0.02 μg/mL GFs,0.2 μg/mL GFs,2 μg/mL GFs,5 μg/mL GFs. GFP-HUVEC was inoculated into the porous plate or gel and cultured for a certain time. Then the proliferation of GFP-HUVEC was detected by Fluorescence microscope or CCK-8. Results(1)HUVEC transfected with GFP was in good condition and showed strong fluorescence under microscope.(2)HUVEC was cultured with different concentrations of growth factors in the hole plate. The cells had the highest proliferative activity at 2 μg/mL PDGF-BB(P < 0.001)and 0.02 μg/mL VEGF-A(P < 0.000 1),respectively.(3)CCK-8 showed the highest cell proliferative activity in2 μg/mL PDGF-BB(P < 0.05)and 0.02 μg/mL VEGF-A(P < 0.001).(4)The cells were encapsulated in the hydrogel and cultured in the medium with different concentrations of growth factors. The cells had the highest proliferative activity at 2 μg/mL PDGF-BB(P < 0.01)and 0.02 μg/mL VEGF-A(P < 0.01),respectively. Conclusion HUVECs respectively had the best effect of cell proliferation in 2 μg/mL PDGF-BB and 0.02 μg/mL VEGF-A. However,high concentrations of growth factors may have an inhibitory effect on them.
作者
魏建国
段东明
井一涵
娄爱菊
曾裕威
刘子恺
王簕
WEI Jianguo;DUAN Dongming;JING Yihan;LOU Aiju;ZENG Yuwei;LIU Zikai;WANG Le(Department of Orthopedics,the Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2021年第23期2977-2983,共7页
The Journal of Practical Medicine
基金
国家自然科学基金(编号:82072409)
广东省自然科学基金(编号:2019A1515012020)。
关键词
生长因子
浓度
细胞增殖
培养环境
growth factors
concentration
cell proliferation
culture environment
