摘要
[目的]探索适宜番茄灰叶斑病人工接种技术体系,并对抗病种质资源进行筛选。[方法]以番茄材料LN89为抗病材料,番茄材料LN163为感病材料,对不同接种方法、接种苗龄、接种孢子浓度、保湿时间进行系统研究。[结果]喷雾接种法是人工接种最适宜的方法,6~8叶期为植株适宜的接种时期,孢子浓度和保湿时间分别为5×10^(4)个/m L,48 h或7×10^(4)个/m L,24 h,并在抗病性鉴定中筛选出3个抗病主栽品种和5个中抗品种。[结论]该研究可为番茄灰叶斑病抗病育种工作的深入开展提供理论依据。
[Objective] The aim was to construct a artificial inoculation system of grey leaf spot on tomato,and screen resistant sources of tomato. [Method] Tomato material LN89 was used as the disease-resistant material,and tomato material LN163 was used as the sensitive material. A systematic study about inoculation method,seedling age,inoculation concentration and moisture preserving time was carried out. [Result] The results showed that spraying inoculation was the proper inoculation method. The seedling age of 6 to 8 leaves was the best inoculation period. The suitable inoculation concentration and the moisture preserving time was 5×10^(4)conidia/mL,48 h or 7×10^(4)conidia/mL,24 h. Spraying inoculation method was used to identify tomato resistant sources. The results indicated that three sources were resistant,five were intermediate resistant and the others were susceptible. [Conclusion] This study provided a theoretical basis for the further development of grey leaf spot on tomato resistance breeding.
作者
张子君
孙亚男
王涛
张逸鸣
邹庆道
ZHANG Zi-jun(Institute of Vegetable Research,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)
出处
《园艺与种苗》
CAS
2021年第11期8-10,共3页
Horticulture & Seed
基金
辽宁省兴辽英才计划项目(XLYC1905009)
辽宁省农业科学院学科建设计划(2019DD041106)。
关键词
番茄
灰叶斑病
人工接种
抗病性鉴定
Tomato
Gray leaf spot
Artificial inoculation
Resistance identification
