摘要
目的:通过网络药理方法探讨散寒祛湿温经通络复方的有效化学成分及作用靶点基因,分析其治疗腰肌筋膜炎的机制。方法:通过TCMSP对散寒祛湿温经通络复方的化学活性物质及作用靶点蛋白进行检索和筛选,应用基因组注释数据库平台(GeneCards)预测疾病的作用靶点,构建活性成分-靶点网络;通过STRING数据库构建蛋白相互作用网络,寻找核心基因,再进行GO富集分析和KEGG富集分析,构建靶点-通路网络。结果:通过筛选,共得到散寒祛湿温经通络复方治疗腰肌筋膜炎的有效成分23个,共同靶点蛋白25个;GO富集分析结果包括抗生素应答、细胞周期蛋白依赖性蛋白激酶全酶复合物、细胞因子受体结合等;KEGG富集分析结果包括AGE-RAGE信号通路、麻疹、内分泌抵抗、炎症性肠病等;得到与上述通路匹配度较高的靶点基因包括IL6、JUN、IL1B、CDK4、CCND1。结论:散寒祛湿温经通络复方可通过调节IL6、JUN、IL1B、CDK4、CCND1等靶点基因对腰肌筋膜炎起到治疗作用。
Objective:To explore the effective chemical constituents and target genes of the Sanhan-Qushi-Wenjing-Tongluo formula through the method of network pharmacology,and to further analyze the mechanism of treatoffing psoas fasciitis.Methods:The TCMSP database was used to search and screen the chemical active substances of Sanhan-Qushi-Wenjing-Tongluo formula and its target proteins acting on the human body.At the same time,the GeneCards database platform was used to predict the target of disease and the active ingredient-target network was constructed.Construct a PPI network through the STRING database,search for PPI core genes,and then perform GO enrichment analysis and KEGG enrichment analysis to find the signal pathways involved and construct a target-path network.Results:Through screening,a total of 23 key chemical components and 25 common target proteins was obtained in Sanhan-Qushi-Wenjing-Tongluo formula treating psoas fasciitis;gene analysis of enrichment analysis results include antibiotic response,cyclin-dependent proteins kinase holoenzyme complex,cytokine receptor binding,etc.Kyoto Encyclopedia of Genes and Genomes enrichment analysis results include AGE-RAGE signaling pathway,measles,endocrine resistance,inflammatory bowel disease,etc;the target genes gained which have a higher degree of matching with the above mentioned pathways include IL6,JUN,IL1B,CDK4,CCND1.Conclusion:Sanhan-Qushi-Wenjing-Tongluo formula could treat psoas fasciitis by regulating the target genes such as IL6,JUN,IL1B,CDK4 and CCND1.
作者
王银平
范鹏
牛宇
郭凤红
赵挺
兰涛
王海龙
刘超
Wang Yinping;Fan Peng;Niu Yu;Guo Fenghong;Zhao Ting;Lan Tao;Wang Hailong;Liu Chao(Rehabilitation Department,Qinhuangdao Hospital of Traditional Chinese Medicine,Qinghuangdao 066000,China)
出处
《国际中医中药杂志》
2021年第11期1124-1130,共7页
International Journal of Traditional Chinese Medicine
基金
秦皇岛市科学技术研究与发展计划项目(201902A162)。
