摘要
对贵州本地选育的花椒优良单株的麻味物质进行分析,运用高效液相色谱法(high performance liquid chromatography,HPLC)对威宁1号~4号花椒优良单株的麻味物质进行测定。研究结果表明:HPLC法测定花椒麻味物质的分离效果好,绘制标准曲线,相关系数≥0.995,且精密度、稳定性、重复性以及加标回收率的RSD均小于1.7%。羟基-α-山椒素的含量较高,在50~57 mg/g,羟基-β-山椒素及羟基-γ-山椒素的含量范围均在3~5 mg/g,说明羟基-α-山椒素是花椒主要的麻味物质,是羟基-β-山椒素及羟基-γ-山椒素的10倍以上。3种麻味物质总含量的大小依次为威宁2号>威宁4号>威宁3号>威宁1号,为贵州花椒特色产业的种植区划及开发利用提供理论依据。
The hemp flavor substances of the excellent individual plant Zanthoxylum bungeanum selected locally in Guizhou were analyzed,and hemp flavor substances of the excellent individual plant Zanthexylum bungeanum of Weining No.1~No.4 were determined by high performance liquid chromatography(HPLC).The research results showed that the HPLC method for the determination of Zanthoxylum bungeanum had a good separation effect.The correlation coefficients of the standard curve≥0.995,and the RSD of precision,stability,repeatability and spike recovery were all less than 1.7%.The content of hydroxyl-α-Zanthoxylin was from 50 to 57 mg/g,which was relatively high.The content of hydroxyl-β-Zanthoxylin and hydroxyl-γ-Zanthoxylin were both in the range of 3 to 5 mg/g,indicating that hydroxyl-α-Zanthoxylin was the main hemp flavor substance of Zanthoxylum bungeanum.It was more than 10 times of hydroxyl-β-zanthoxylin and hydroxyl-γ-zanthoxylin.The total contents of the three hemp flavor substances were in the order of Weining 2>Weining 4>Weining 3>Weining 1,which provided theoretical basis for the planting division,development and utilization of guizhou Zanthoxylum bungeanum characteristic industry.
作者
杨娴
曾钦朦
罗红
侯娜
王港
YANG Xian;ZENG Qin-meng;LUO Hong;HOU Na;WANG Gang(Liupanshui Forestry Bureau,Liupanshui 5530009 Guizhou,China;Guizhou Academy of Forestry Sciences,Guiyang 550000,Guizhou,China)
出处
《粮食与油脂》
北大核心
2021年第12期153-157,共5页
Cereals & Oils
基金
国家林业局林业公益性行业科研专项(201304706)
西北农林科技大学产业技术集成与示范推广(TGZX201608)
贵州省科技厅中药现代化项目(黔科合ZY字[2012]3002号)
贵州省科技厅优秀青年科技人才项目(黔科合平台人才[20195643])
贵州省科技厅科技支撑项目(黔科合支撑[2021]222号)
贵州省林业厅项目(黔林科合2018【01】)
贵州本地花椒优良种质资源收集保存及良种选育(特林研2020-18)
贵州花椒高效栽培与主要病虫害绿色防控技术研究与示范(特林研2020-22)。
关键词
花椒
优良单株
麻味物质
标准曲线
高效液相色谱法
Zanthoxylum bungeanum
excellent individual plant
hemp flavor substance
standard curve
high performance liquid chromatography(HPLC)
