摘要
小黄鱼(Larimichthys polyactis)是我国近海重要的经济和优势鱼种,在食物网结构中起承上启下的关键作用。利用气相色谱质谱联用仪测定了浙江南部近海小黄鱼的肌肉脂肪酸含量并分析其组成变化特点,基于特征脂肪酸的食源指示功能开展小黄鱼的食性分析。结果表明:小黄鱼肌肉中共检测出30种脂肪酸,主要脂肪酸包括C16∶0、C17∶0、C18∶1n9c、C18∶3n3、C16∶1n7、C20∶1、C20∶4n6、C20∶5n3(EPA)和C22∶6n3(DHA)等,其中C16∶0含量最高(23.12%),其次为C18∶1n9c(16.67%)、C16∶1n7(11.45%)和C22∶6n3(11.40%);肌肉中不饱和脂肪酸(UFA)平均含量达到60.20%,显著高于饱和脂肪酸(SFA)的含量(39.80%),DHA和EPA在机体中含量占比达15.54%,体现出小黄鱼肌肉具有较高营养价值;双因素方差分析显示饱和脂肪酸含量在体长组间存在显著差异,含量随着体长的增大而减小,而单不饱和脂肪酸(MUFA)含量存在显著的季节性差异;绝大多数特征脂肪酸在各季节的体长组间无显著差异,而120~139 mm体长组和>180 mm体长组的C16∶1n7、C20∶1和C18∶1n9c在冬季的含量显著小于其他季节;基于脂肪酸食源指示功能,小黄鱼属于浮游、底栖与游泳动物混合食性的鱼类,初始碳源包括浮游硅藻类、甲藻类和底栖生物等。本研究利用了特征脂肪酸稳定指示食物来源的特点,进一步补充和验证文献资料中小黄鱼的胃含物分析结果,为小黄鱼的摄食生态学研究提供重要参考。
Small yellow croaker(Larimichthys polyactis) is an important economic species and the dominant species in China’s coastal waters,playing a key role in the structure of food web.The contents of muscle fatty acids from 58 small yellow croakers were determined by gas chromatography-mass spectrometer and the characteristics of their changes in composition were analyzed.The feeding habits of yellow croaker were investigated based on the role of fatty acids as dietary tracers.The results showed that 30 kinds of fatty acids were detected in the muscle of small yellow croaker,with prevalent fatty acids C16∶0,C17∶0,C18∶1 n9 c,C18∶3 n3,C16∶1 n7,C20∶1,C20∶4 n6,C20∶5 n3(EPA),C22∶6 n3(DHA),among which the C16∶0 accounted for 23.12%,C18∶1 n9 c for 16.67%,C16∶1 n7 for 11.45% and C22∶6 n3 for 11.40%.The average content of unsaturated fatty acid(UFA) in muscle reached 60.20%,which was significantly higher than that of saturated fatty acid(SFA,39.80%),and the proportion of DHA and EPA in muscle accounted for 15.54%,indicating that the fish had higher nutritional value.Two-way ANOVA showed that there was a significant difference in saturated fatty acid SFA among body length groups,and the content decreased with the increase of body length(P<0.05),while the content of monounsaturated fatty acid MUFA had significant seasonal differences(P<0.05).Most of the characteristic fatty acids had no significant difference among body length groups in each season(P>0.05),while the contents of C16∶1 n7,C20∶1 and C18∶1 n9 c in 120-139 mm body length group and > 180 mm body length group in winter were significantly lower than those in other seasons.The fatty acid signatures,known as trophic markers,revealed that small yellow croaker was a kind of fish that feeds on plankton,benthic and swimming animals,particularly on diatoms,dinoflagellates,zooplankton and benthic organisms.This highlights the fatty acid markers to indicate the food source of small yellow croaker,supporting and verifying the results of contents of
作者
王腾
高春霞
王少琴
韩东燕
叶深
WANG Teng;GAO Chunxia;WANG Shaoqin;HAN Dongyan;YE Shen(College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,China;National Engineering Research Center for Oceanic Fisheries,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources,Shanghai Ocean University,Shanghai 201306,China;Scientific Observing and Experimental Station of Oceanic Fishery Resources,Ministry of Agriculture and Rural Affairs,Shanghai 201306,China;Zhejiang Mariculture Research Institute,Wenzhou 325005,Zhejiang,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2021年第6期992-1001,共10页
Journal of Shanghai Ocean University
基金
国家自然科学基金(31902372,41906074)
上海海洋大学青年教师科研启动基金(210021)
温台渔业资源专项调查项目(158053)。
关键词
小黄鱼
脂肪酸
食源指示
浙江南部近海
small yellow croaker
fatty acid
dietary indicator
offshore waters of southern Zhejiang
