摘要
目的探讨肝细胞癌病人线粒体DNA拷贝数与临床病理特征的关系及对预后的影响方法采用回顾性队列研究方法。收集2011年3--6月海军军医大学附属东方肝胆外科医院收治的71例行手术切除肝细胞癌病人的临床病理资料;男61例,女10例;中位年龄为55岁.年龄范围为26-80岁。所有病人检测肝癌组织和癌旁组织线粒体DNA拷贝数。观察指标:(1)肝细胞癌病人肝癌组织和癌旁组织线粒体DNA拷贝数及其与临床病理特征的关系(2)随访情况。(3)肝细胞癌病人预后影响因素分析。采用门诊或电话方式进行随访。了解病人术后生存情况。随访时间截至2019年9月正态分布的计量资料以x土8表示,组间比较采用独立样本1检验或配对样本1检验。偏态分布的计量资料以M(范围)表示。计数资料以绝对数表示,组间比较采用》检验或Fisher确切概率法。单因素和多因素分析均采用COX回归模型,单因素分析中P<0.10的指标纳入多因素分析。采用Kaplan-Meier法计算生存率和绘制生存曲线.采用Log-rank检验进行生存分析。结果(1)肝细胞癌病人肝癌组织和癌旁组织线粒体DNA拷贝数及其与临床病理特征的关系:71例肝细胞癌病人肝癌组织线粒体DNA拷贝数为0.85+0.08.癌旁组织线粒体DNA拷贝数为1.16+0.08.两者比较.差异有统计学意义(1=2.96.P<0.05)。71例病人中.48例为线粒体DNA低拷贝数,23例为线粒体DNA高拷贝数。线粒体DNA低拷贝数和高拷贝数病人肿瘤包膜(完整、不完整)和微血管侵犯(有、无)分别为20.28例.21、27例和16.7例.4、19例,上述指标比较.差异均有统计学意义(x^(2)=4.84.4.74.P<0.05)。(2)随访情况:71例病人均获得随访.随访时间为2.1~85.3个月,中位随访时间为47.8个月。71例病人1.3.5年总体生存率分别为87.39%6.64.7%.37.4%。48例线粒体DNA低拷贝数病人1.3.5年总体生存率分别为81.2%.50.0%.29.2%;23例线粒体DNA高拷贝数病人1.3.5年总体�
Objective To investigate the relationship of mitochondrial DNA(mtDNA)copy number with clinicopathologic characteristics and its influence on the prognosis of hepatocellular carcinoma(HCC)patients.Methods The retrospective case-control study was conducted.The clinicopathological data of 71 HCC patients undergoing surgical treatment in the Eastern Hepatobiliary Surgery Hospital of Naval Medical University from March to June 2011 were collected.There were 61 males and 10 females,aged from 26 to 80 years,with a median age of 55 years.The mtDNA copy number of tumor tissues and adjacent normal tissues were measured for all patients.Observation indicators:(1)the mtDNA copy number of tumor tissues and adjacent normal tissues and relationship between the mtDNA copy number and clinicopathological characteristics of HCC patients;(2)follow-up;(3)related factors for the prognosis of HCC patients.Follow-up using outpatient examination or telephone interview was conducted to detect postoperative survival of patients up to September 2019.Measurement data with normal distribution were described as Mean±SD,and comparison between groups was analyzed using independent samples t test or the matched samples t test.Measurement data with skewed distribution were described as M(range).Count data were represented as absolute numbers,and comparison between groups was analyzed using the chi-square test or Fisher exact probability.Univariate and multivariate analyses were conducted using the COX regressional model.Variables with P<0.10 in the univariate analysis were included for the multivariate analysis.Survival rates were calculated using the Kaplan-Meier method,and Log-rank test was used for survival analysis.Results(1)The mtDNA copy number of tumor tissues and adjacent normal tissues and relationship between the mtDNA copy number and clinicopathological characteristics of HCC patients:of 71 HCC patients,the mtDNA copy number was 0.85+0.08 in tumor tissues,versus 1.16+0.08 in adjacent normal tissues,showing a significant difference betw
作者
陈芳芳
彭祖祥
皮儒先
梁先春
唐文
王海波
唐春
Chen Fangfang;Peng Zuxiang;Pi Ruxian;Liang Xianchun;Tang Wen;Wang Haibo;Tang Chun(Department of Oncology,Chongqing General Hospital,University of Chinese Academy of Sciences,Chongqing 400013,China;Department of Hepatobiliary Pancreatic Surgery,Doping Hospital,Army Medical Center of PLA,Chongqing 400042,China;The Fourth Department of Hepatic Surgery,the Third Affiliated Hospital of Naval Medical University,Shanghai 200433,China)
出处
《中华消化外科杂志》
CAS
CSCD
北大核心
2021年第12期1306-1312,共7页
Chinese Journal of Digestive Surgery
基金
上海市博士后科研资助计划面上项目(13K21418400)。
关键词
肝肿瘤
线粒体
脱氧核糖核酸
病理学
预后
分析
Liver neoplasms
Mitochondrial
Deoxyribonucleic acid
Pathology
Prognosis
Analysis
