摘要
目的探讨化学发光法在监测新型冠状病毒疫苗注射后机体产生抗体的效果及鉴别胶体金免疫层析法(金标法)假阳性中的应用价值。方法注射新型冠状病毒疫苗的志愿者22例,分别在注射第2剂后第1、3、6个月采集静脉血。每例患者标本用化学发光法检测新型冠状病毒抗体免疫球蛋白(Ig)M、IgG、IgA、总抗体(TAb)和中和抗体(NAb),同时用金标法检测新型冠状病毒抗体IgM和IgG。另外,用化学发光法复核74例金标法检测新型冠状病毒抗体IgM或IgG疑为阳性者(核酸检测均阴性)的上述5种抗体。结果在注射新型冠状病毒疫苗后1、3、6个月,化学发光法检测的IgG阳性率均明显高于两种金标法试剂(P<0.05);第3个月和6个月时,除IgA、TAb,化学发光法检测的其他抗体水平和阳性率均分别明显低于第1个月(P<0.05);化学发光法检测的IgG、TAb和NAb阳性率均从第1个月的90.00%分别降低到第6个月的36.36%、63.64%和31.82%;TAb和IgG分别与NAb显著相关(r=0.873,P<0.001;r=0.862,P=0.001);74例金标法筛查疑为阳性者中,化学发光法复核后阳性者仅9例,此9例多数为两种或两种以上抗体阳性,且均为疫苗接种者或既往感染者。结论化学发光法在监测新型冠状病毒疫苗接种后抗体的产生上,在疫苗接种后1个月和6个月时,分别有95.00%和63.64%个体至少可检测到5种抗体中的1种,检测IgG和TAb基本可以反映NAb的水平。化学发光法可用于鉴定金标法筛查疑为新型冠状病毒抗体阳性的标本。
Objective To investigate the effect of chemiluminescent method in monitoring antibody production after vaccine injection and its use for the identification of false positive results by colloidal gold immunochromatography.Methods Venous blood samples were collected from 22 volunteers who received SARS-CoV-2 vaccine at the end of 1st,3rd and 6th month after the second dose.Each sample′s immunoglobulin(Ig)M,IgG,IgA,neutralizing antibody(NAb)and total antibody(TAb)against SARS-CoV-2 were determined simultaneously by chemiluminescent method,and IgM and IgG were determined by colloidal gold immunochromatography method.Furthermore,74 cases with suspected positive IgM or IgG results were re-tested by chemiluminescent method(all sample′s SARS-CoV-2 nucleic acids were negative).Results The positive rates of IgG detected by chemiluminescent method at the end of 1st,3rd,and 6th month after the second dose were significantly higher than those of either colloidal gold immunochromatography method regent(P<0.05).At the end of 3rd and 6th month,except IgA and TAb,both levels and positive rates of other antibodies were significantly lower than those at the end of 1st month(P<0.05).The positive rates of IgG,TAb and NAb detected by chemiluminescent decreased from 90.00%at the 1st month to 36.36%,63.64%and 31.82%at end of the 6th month respectively.TAb and IgG level significantly correlated with NAb(r=0.873,P<0.001;r=0.862,P=0.001).Among the 74 cases suspected to be positive by colloidal gold immunochromatography method,only 9 cases were proved positive by chemiluminescent method(most were positive for two or more types of antibodies),and all of them were received SARS-CoV-2 vaccine or previous infected by the virus.Conclusion The chemiluminescent method is used in monitoring antibody production after SARS-CoV-2 vaccination at the end of 1st month and 6th month after vaccination,95.00%and 63.64%of the individuals could be observed at least one type of antibody.IgG or TAb level could basically reflect the level of NAb,chemilumines
作者
刘淼
赵思蒙
董一红
张岩
车冬丽
田野
李卓敏
李杰
吴吉兵
蔡嘉镜
谭延国
LIU Miao;ZHAO Simeng;DONG Yihong;ZNANG Yan;CHE Dongli;TIAN Ye;LI Zhuomin;LI Jie;WU Jibing;CAI Jiajing;TAN Yanguo(Department of Clinical Laboratory,Fuxing Hospital,Capital Medical University,Beijing 100038,China;Department of Clinical Laboratory,the Fourth Medical Center,Chinese PLA General Hospital,Beijing 100142,China;Department of Clinical Laboratory Diagnosis,Capital Medical University,Beijing 100038,China;Department of Emergency,Fuxing Hospital,Capital Medical University,Beijing 100038,China;Maccura Biotechnology Co.,Ltd,Chengdu,Sichuan 611731,China)
出处
《国际检验医学杂志》
CAS
2021年第24期2945-2948,2952,共5页
International Journal of Laboratory Medicine
基金
北京市临床重点专科医学检验科培育项目[京卫医(2020)129号]。
