摘要
目的:制定金铁锁种子质量检验方法,建立金铁锁种子分级标准。方法:参照《农作物种子检验规程》(GB/T 3543.3—1995),建立金铁锁种子真实性、净度、千粒质量、含水量、生活力、发芽率检验方法,通过K-均值聚类分析36个不同产地金铁锁种子,制定质量分级标准。结果:金铁锁种子净度分析样品应不少于10 g,送检样品应不少于100 g;种子真实性鉴定应采用外观形态观察法及外观形态测量法;种子质量测定应采用千粒法;种子含水量测定采用(130±2)℃恒温烘干4 h;生活力测定采用2,3,5-三苯基氯化四氮唑(TTC)染色法,最佳条件为30℃下0.01%TTC染色6 h;发芽率测定应采用滤纸发芽床,经2.5%NaClO浸泡10 min后,于20℃恒温光照培养,发芽计数时间为4~16 d。不同来源合格金铁锁种子可分为3个等级,以发芽率和生活力为质量分级标准的主要指标、千粒质量和净度为参考指标。结论:本研究制定的金铁锁种子质量分级标准和等级评定方法科学、可行,可为金铁锁种子质量评价提供参考。
Objective:To determine the quality test methods and establish the grading standard for Psammosilene tunicoides seeds.Methods:The test methods for sampling amount,authenticity,purity,seed weight,moisture content,viability,and germination rate of P.tunicoides seeds were established according to the Rules for Agricultural Seed Testing(GB/T 3543.3—1995).The quality of seeds from 36 producing areas was determined for the formulation of quality grading standard by K-means clustering.Results:At least 100 g seeds should be sampled for testing and no less than 10 g for purity analysis.Seed weight should be measured based on the 1000-seed weight.For the moisture content,seeds should be dried at(130±2)℃for 4 h.The viability should be measured by 0.01%2,3,5-triphenyltetrazolium chloride(TTC)staining(30℃,6 h).In terms of the germination rate,seeds should be first soaked in 2.5%NaClO for 10 min and then cultured on filter paper(20℃,in light,4-16 days).Seeds from 36 producing areas were classified into three grades with the main indexes of germination rate and viability and reference indexes of 1000-seed weight and purity.Conclusion:The seed quality grading standard and quality testing methods were scientific and viable,which can serve as a reference for quality evaluation of P.tunicoides seeds.
作者
熊永兴
曾宇君
苏钛
李云
周国华
杨成金
XIONG Yong-xing;ZENG Yu-jun;SU Tai;LI Yun;ZHOU Guo-hua;YANG Cheng-jin(Yunnan Institute of Materia Medica/Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation,Kunming 650111,China;Yunnan Baiyao Group Chinese Medicinal Resources Co.,Ltd.,Kunming 650111,China;Yunnan Baiyao Group Pharmaceutical Co.,Ltd.,Lijiang 674100,China)
出处
《中国现代中药》
CAS
2021年第11期1942-1948,共7页
Modern Chinese Medicine
基金
云南省应用基础研究计划项目(201501YH00081)。
关键词
金铁锁种子
质量检验
分级标准
Psammosilene tunicoides seeds
quality test
grading standard
