摘要
为探究盐度胁迫对贝类的影响,以河蚬(Corbicula fluminea)为对象,研究不同盐度胁迫下河蚬的摄食率以及鳃Na^(+)/K^(+)-ATP酶、Ca^(2+)-ATP酶和Mg^(2+)-ATP酶活力的变化规律,评估河蚬的耐盐性能,筛选适宜评价淡水双壳贝类耐盐性能的指示指标。结果显示,盐度5、15和25组河蚬摄食率均出现极显著下降拐点(P<0.01),分别为2.11×10^(8)、1.09×10^(8)、7.68×10^(7)个·(g·h)^(-1);盐度5和15组,鳃3种ATP酶活力在4 d时均达到最大值(P<0.01),鳃Na^(+)/K^(+)-ATP酶与Mg^(2+)-ATP酶第5天恢复到初始值附近(P>0.05),鳃Ca^(2+)-ATP酶第7天恢复到初始值附近(P>0.05)。盐度25组鳃3种ATP酶活力4 d与对照组相比无显著上升现象(P<0.05)。盐度25组胁迫7 d时出现大量死亡,存活率仅19%。推测盐度5对河蚬产生胁迫,盐度15附近是其高盐耐受临界值。摄食率变化与河蚬耐盐性能存在一定相关性,当摄食率下降超过50%时,贝类开始出现死亡现象,提示该盐度已超出贝类盐度耐受范围,因此摄食率可作为贝类耐盐性能的指示指标;鳃Na^(+)/K^(+)-ATP酶若4 d内不出现活力上升,预示已超过河蚬的耐盐极限,亦可作为贝类耐盐性能指示指标。
In order to explore the effects of salinity stress on shellfish,the ingestion rate and branchial Na^(+)/K^(+)-ATPase,Ca^(2+)-ATPase and Mg^(2+)-ATPase activity of C.fluminea exposed to different acute salinity stresses were investigated.The paper is to evaluate the salinity tolerance of Corbicula fluminea and select the potential indicators for evaluating the salinity tolerance of freshwater bivalves.C.fluminea’s ingestion rate decreased significantly(P<0.01)during 7 days of salinity stress exposure.They were 2.11×10^(8) cells·g^(-1) for treatment of salinity 5,1.09×10^(8) cells/(g·h)for treatment of salinity 15,7.68×10^(7) cells/(g·h)for treatment of salinity 25.In the treatment of salinity 5 and 15,the activity of three branchial ATPases reached the maximum on 4 d(P<0.01),Na^(+)/K^(+)-ATPase and Mg^(2+)-ATPase activity returned to the initial value from 5 d(P>0.05),while Ca^(2+)-ATPase activity returned to the initial value on 7 d(P>0.05).The activity of three branchial ATPases of salinity 25 treatment did not increase on 4 d,and no significant difference was found compared to the control(P<0.05).The survival rate of C.fluminea in salinity 25 treatment was 19%.It was indicated that salinity 5 was the start of salinity stress-point for C.fluminea,the highest tolerance salinity was about 15.Certain correlation between the ingestion rate and the salinity tolerance of C.fluminea indicated that ingestion rate was a potential indicator for evaluating the salinity tolerance of bivalves.Stability of branchial Na^(+)/K^(+)-ATPase activity within 4 d could also be a potential indicator for salinity tolerance.
作者
包锬
刘一萌
来琦芳
么宗利
周凯
高鹏程
BAO Tan;LIU Yimeng;LAI Qifang;YAO Zongli;ZHOU Kai;GAO Pengcheng(College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Engineering Research Center for Saline-alkaline Fisheries, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200090, China)
出处
《海洋渔业》
CSCD
北大核心
2021年第6期671-679,共9页
Marine Fisheries
基金
国家重点研发计划(2020YFD0900401)
中国水产科学研究院东海水产研究所基本科研业务费资助“盐碱水养殖贝类筛选与评估”(课题号2018 Z02-02)。
关键词
河蚬
鳃
ATP酶
摄食率
Corbicula fluminea
gill
ATPase
ingestion rate
