摘要
目的:比较分析不同方法鉴定包装饮用水中铜绿假单胞菌含量。方法:采用GB8538.57—2016、API 20E鉴定及VITEK鉴定3种方法对25株野生型菌株进行鉴别,使用16SrRNA基因序列分析与3种鉴别结果进行比对。结果:GB8538.57—2016鉴定结果与16SrRNA基因序列对比结果一致,API 20E鉴定5株为假阳性菌株,占比20.0%;VITEK鉴定3株为假阳性菌株,占比12.0%。结论:鉴定包装饮用水中铜绿假单胞菌需将GB8538.57—2016、API 20E鉴定及VITEK鉴定互相结合,辅佐印证,提高鉴别准确性。
Objective:To explore the identification of Pseudomonas aeruginosa in packaged drinking water by different methods.Methods:25 wild-type strains were identified by GB8538.57—2016,API 20E and VITEK.16SrRNA Gene Sequence analysis was used to compare with three identification results.Results:The identification results of GB8538.57—2016 were consistent with the comparison results of 16SrRNA gene sequence.Five strains were identified as false positive by API 20E,accounting for 20.0%.Three strains were identified as false positive by Vitek,accounting for 12.0%.Conclusion:The identification of Pseudomonas aeruginosa in packaged drinking water should be combined with GB8538.57—2016,API 20E identification and VITEK identification,assist in confirmation and improve the accuracy of identification.
作者
赵端阳
阎学燕
陈磊
ZHAO Duan-yang;YAN Xue-yan;CHEN Lei(Clinical laboratory,Kaifeng Center for Disease Control and Prevention,Henan 475000 China)
出处
《疾病监测与控制》
2021年第5期352-354,共3页
Journal of Diseases Monitor and Control
关键词
包装饮用水
铜绿假单胞菌
假阳性
packaged drinking water
pseudomonas aeruginosa
a false positive
