摘要
运用同位素标记相对和绝对定量(iTRAQ)技术,研究了以恒温冻藏和常规冻藏2种方式分别贮藏新鲜中华管鞭虾30,60,90 d后肌肉蛋白的表达差异,探索差异蛋白的分布、功能和虾在冻藏过程中的品质变化机制。结果表明:与新鲜中华管鞭虾相比,恒温冻藏组中分别有79,105,125个差异蛋白;常规冻藏组中分别有100,125,141个差异蛋白。根据试验结果筛选出肌球蛋白重链和肌质钙结合蛋白2个鲜度指示蛋白。
Using iobaric tags for relative and absolute quantification(iTRAQ)technology,the expression of differential proteins in muscular of Solenocera melantho stored in constant temperature and conventional frozen storage group for 30,60 and 90 days was studied,and the distribution and function of differential protein and the quality change mechanism of Solenocera melantho during frozen storage were explored.The results showed that there were 79,105 and 125 differential proteins in the constant temperature frozen storage group,and 100,125 and 141 differential proteins in the conventional frozen storage group,respectively,compared with fresh Solenocera melantho.Two freshness indicator proteins,including myosin heavy chain and sarcoplasmic calcium-binding protein were screened out based on the test results.
作者
石娟
李勇勇
周丽萍
李超群
王晴
娄永江
Shi Juan;Li Yongyong;Zhou Liping;Li Chaoqun;Wang Qing;Lou Yongjiang(College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo 315211,Zhejiang)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第11期120-132,共13页
Journal of Chinese Institute Of Food Science and Technology
基金
国家重点研发计划项目(2020YFD0900900,2020YFD0900903)。
关键词
ITRAQ
中华管鞭虾
差异蛋白
冻藏
鲜度指示蛋白
iTRAQ
Solenocera melantho
differential proteins
frozen storage
freshness indicator proteins
