摘要
目的探讨TESA获得的稀少精子冻融后行ICSI的受精结局。方法回顾性分析本中心2014年1月至2021年4月对因严重少、弱、畸形精子症患者射出的精子行常规ICSI,对非梗阻性无精子患者经过TESA得到的少量新鲜或冻融后精子行ICSI,共200例患者为研究对象。按精子来源不同分为A:常规ICSI精子组(n=107);B:冷冻TESA精子组(n=35);C:新鲜TESA精子组(n=58)。通过病例对照研究,分析比较各组临床一般资料和实验室数据,评估胚胎质量。结果B组获卵数、卵子成熟率[(11.63±2.87)枚,82.31%]与A组[(10.81±3.21)枚,82.63%]和C组[(11.46±3.16)枚,82.41%]相比,差异无统计学意义(P>0.05)。B组的正常受精率、正常卵裂率、优质胚胎率(62.99%、84.83%、34.64%)与A组(82.11%、97.07%、48.29%)和C组(73.18%、91.02%、42.47%)相比,差异有统计学意义(P<0.05)。结论经皮睾丸精子抽吸术获得的稀少精子冷冻复苏后行卵胞浆内单精子注射能够解决非梗阻性无精子症患者的不育难题。
Objective To investigate the fertilization outcome of intracytoplasmic sperm injection(ICSI)after freezing and thawing of rare sperm obtained by testicular puncture(TESA)in patients with non-obstructive azoospermia.Methods A total of 200 patients,including the patients with severe oligozoospermia,asthenozoospermia,and teratozoospermia who underwent conventional ICSI on ejaculated sperm and the patients with non-obstructive azoospermia ICSI who underwent ICSI on a small amount of fresh or frozen-thawed sperm obtained by TESA from January 2014 to April 2021 in our center were retrospectively analyzed.They were divided into group A(conventional ICSI sperm group,107 cases),group B(frozen TESA sperm group,35 cases),group C(fresh TESA sperm group,58 cases)according to different sperm sources.Through case-control studies,the general clinical data and laboratory data of each group were analyzed and compared.The embryo quality was evaluated.Results There was no significant difference in the number of egg retrieved and egg maturation rate between group B[(11.63±2.87),82.31%]and group A(10.81±3.21,82.63%)and group C(11.46±3.16,82.41%)(P>0.05).The normal fertilization rate,normal cleavage rate and high-quality embryo rate of group B(62.99%,84.83%,34.64%)were significantly different from those of group A(82.11%,97.07%,48.29%)and group C(73.18%,91.02%,42.47%)(P<0.05).Conclusion Intracytoplasmic sperm injection after cryopreservation of rare sperm obtained by testicular sperm aspiration can solve the infertility problem in patients with non-obstructive azoospermia.
作者
马文叶
程方
刘芳
康卉娴
唐大伟
白刚
任萌萌
金锐
MA Wenye;CHENG Fang;LIU Fang;KANG Huixian;TANG Dawei;BAI Gang;REN Mengmeng;JIN Rui(Reproductive Center,Yinchuan Maternity and Child Health Care Hospital,Yinchuan 750001,China)
出处
《中国现代医生》
2021年第29期32-35,共4页
China Modern Doctor
