摘要
目的通过生物信息学分析建立结直肠癌竞争性内源RNA(ceRNA)网络,对筛选出的ceRNA C14orf132-GAS1的表达及意义进行探讨。方法基于肿瘤基因组图谱(TCGA)数据库对结直肠癌数据集进行分析,筛选差异表达的长非编码RNA(lncRNA)和mRNA,采用倍数法(fold change)和t检验识别差异表达基因,校正后P<0.05且|log2 fold change|>1的基因被鉴定为差异表达基因。采用超几何检验方法筛选出lncRNA-mRNA形成的ceRNA对,并对可能的靶微小RNA(miRNA)进行筛选。从TCGA数据库下载结直肠癌临床数据集。使用R包的"survival"和"survminer"进行生存分析。选取30例新鲜的结直肠癌及癌旁组织,应用荧光实时定量聚合酶链反应(RT-qPCR)对筛选出的ceRNA对和miRNA进行检测,并对这些分子间的相关关系进行Pearson相关分析。结果肿瘤组织中上调的mRNA数量为1731,下调的为3691;上调的lncRNA为1126,下调的为2105;上调的miRNA为14,下调的为190。经过筛选,确定C14orf132-miR-33a-GAS1轴可能为结直肠癌进展中有重要意义的ceRNA。生物信息学结果显示,C14orf132-GAS1作为ceRNA对,在肿瘤中表达水平(13.37±1.76、14.45±4.55)低于正常组织(15.71±0.62、16.00±2.42,t=-9.44、-2.40,P<0.05);miR-33a在肿瘤中表达水平(5.53±1.84)高于正常组织(3.25±0.77,t=4.10,P<0.01)。组织验证结果与生物信息学结果符合。结论由C14orf132-miR-33a-GAS1轴形成的ceRNA机制可能在结直肠癌进展过程中发挥重要作用。
Objective A colorectal cancer competitive endogenous RNA(ceRNA)network was established,and the expression and significance of the selected ceRNA C14orf132-GAS1 were explored via bioinformatics analysis.Methods The colorectal cancer data set was analyzed based on the cancer genome atlas(TCGA)database,and screen the differentially expressed long non-coding RNA(lncRNA)and mRNA.After correction,fold change and t test was used to identify differentially expressed genes,genes with P<0.05 and|log2 fold change|>1 were identified as differences Express genes.The hypergeometric test was used to screen ceRNA pairs formed by lncRNA-mRNA,and to screen potential target microRNAs(miRNAs).The colorectal cancer clinical data set from the TCGA database was downloaded.The"survival"and"survminer"of the R package was used for survival analysis.30 cases of fresh colorectal cancer and adjacent tissues were selected,and fluorescent real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the selected ceRNA pairs and miRNAs,and the correlation between these molecules were analyzed.Results The number of up-regulated mRNAs in tumor tissues was 1731 and down-regulated was 3691;up-regulated lncRNA was 1126,down-regulated 2105;up-regulated miRNA was 14,and down-regulated was 190.After screening,it was determined that the C14orf132-miR-33a-GAS1 axis might be an important ceRNA in the progression of colorectal cancer.After screening,it was determined that the C14orf132-miR-33a-GAS1 axis might be an important ceRNA in the progression of colorectal cancer.Results of bioinformatics showed that C14orf132-GAS1 as a ceRNA pair,the expression level in tumors(13.37±1.76,14.45±4.55)was lower than that in normal tissues(15.71±0.62,16.00±2.42,t=-9.44,-2.40,P<0.05);the expression level of miR-33a in tumors(5.53±1.84)was higher than that in normal tissues(3.25±0.77,t=4.10,P<0.01).The results of the organization verification were consistent with the results of bioinformatics.Conclusion The ceRNA mechanism formed by the C14orf132-miR-
作者
任维聃
刘桂伟
冶浩鹏
姜辉
郭庆金
耿学辰
姜国胜
Ren Weidan;Liu Guiwei;Ye Haopeng;Jiang Hui;Guo Qingjin;Geng Xuechen;Jiang Guosheng(Department of Colorectal and Anal Surgery,Cangzhou Central Hospital,Cangzhou 061000,China;Department of Anorectal,Cangzhou People′s Hospital,Cangzhou 061000,China)
出处
《中华实验外科杂志》
CAS
北大核心
2021年第12期2495-2498,共4页
Chinese Journal of Experimental Surgery
基金
河北省卫生健康委员会科研基金项目 (20210955)。
